麻疹疫苗及加佐剂经滴鼻免疫小鼠的抗体应答

Antibody Response in Mice to Intranasal Immunization with Attenuated Measles Vaccine with or without Adjuvants

目的:观察麻疹减毒活疫苗和灭活疫苗经滴鼻免疫小鼠后的抗体应答。方法:用活的或灭活麻疹 疫苗及分别加明胶、植物血凝素(PHA)的疫苗滴鼻免疫小鼠,于末次免疫后10d取血清、唾液、呼吸道分泌物, 用间接ELISA法检测特异性IgG,IgA抗体水平,并与皮下注射组及对照组进行比较。结果:各滴鼻组小鼠血清 及呼吸道分泌物中均产生一定水平的特异性IgG及IgA抗体,皮下注射组小鼠呼吸道分泌物中未产生特异性 IgA抗体;加明胶后的免疫效果优于加PHA的。结论:麻疹疫苗滴鼻免疫小鼠后可诱导全身及局部抗体应答, 明胶可增强其免疫效果,而麻疹疫苗皮下注射免疫小鼠未能诱导局部抗体应答。

Objective: To survey the antibody response of mice to intranasal immunization of alive and attenuated measles vaccines. Methods: The mice were immunized by intranasal with alive or inactive measles vaccines with or without glutin or phytohemagglutinin (PHA). The sera and saliva and secretion of trachea were collected in 10 days after immunization. The IgG and IgA against measles virus were assayed by indirect ELISA test. Results: Specific IgG and IgA were found in sera and tracheal secretion of mice when immunized intranasally. But, for those mice that immunized by subcutaneous infection, the specific IgG and IgA can be found only in sera, not in tracheal secretion. Glutin showed better synergy effect to the vaccine than PHA. Conclusions: Intranasal immunization of measles vaccine can induce both local and system antibody response. However, subcutaneous immunization can not induce local antibody response. Glutin can increase the local antibodiy response.