逆癌酮抗小鼠肺癌作用的初步研究

A preliminary study of antineoplastic effects of Anticancer Ketonon on lung cancer in mice

背景与目的 研究发现丹参酮具有抗肿瘤作用,逆癌酮是以丹参酮为主要成分并加入其它抗肿 瘤成分的中药制剂。本研究拟探讨逆癌酮对小鼠Lewis肺癌的抑癌作用及其机制。方法 用逆癌酮进行体 内抑癌实验,并用流式细胞术检测肿瘤组织中细胞凋亡指数和细胞周期分布。结果 共进行两次实验,每次 实验单一性别小鼠。逆癌酮组与5 Fu组的瘤重与对照组比较均有显著性差异(P<0.05),逆癌酮对小鼠 Lewis肺癌的抑瘤率分别为38.9%和32.2%,5 Fu对小鼠Lewis肺癌的抑瘤率分别为59.6%和53.9%;各 组间肺部转移率均无显著性差异(P>0.05)。流式细胞仪分析表明,逆癌酮组细胞凋亡指数(11.22%)明显 高于对照组(5.43%)(P<0.05),而细胞周期分布与对照组比较无显著性差异(P>0.05)。结论 逆癌酮对 小鼠Lewis肺癌有抑癌作用,它可能是通过诱导肿瘤细胞凋亡实现的。

Background and objective It has been proved that Tanshinone has obvious anticancer effect, but its mechanisms of anticancer are still unknown. Anticancer Ketonon is complex antitumor drug which Tanshinone is combined with other anticancer elements. This study aims to explore the antineoplastic effects of Anticancer Ketonon on Lewis lung cancer and the mechanisms in mice. Methods The mice were divided into three groups: Ketonon group, 5-fluorouracil (5-Fu) group and control group. The former two groups were treated with responsive drugs after subcutaneous inoculation of Lewis lung cancer. The last group was only treated with normal saline after inoculation. Apoptosis index and cell cycle were measured by flow cytometry. Results Two experiments were carried out in male and female mice respectively. The tumor inhibitory rates of Anticancer Ketonon were 38.9% and 32.2% respectively, those of 5-FU were 59.6% and 53.9%. Compared with those of control groups, the tumor weights in Ketonon group and 5-Fu group were statistically decreased (P< 0.05). Metastasis rates of the lung in the three groups were not statistically different (P>0.05). The apoptosis index of Ketonon group was significantly higher than that of control group (P<0.05), but the cell cycle was not statistically changed compared with that of control group (P>0.05). Conclusion Anticancer Ketonon has antineoplastic effect on Lewis lung cancer in mice and the mechanism may be associated with inducing apoptosis of tumor cells.