肺癌患者深部真菌感染主要病原菌的FCM分析

Analysis of main pathogen of deep fungal infection in patients with lung cancer by FCM

背景与目的 深部真菌感染是晚期肺癌患者的重要并发症和致死原因之一,研究和分析肺癌患 者深部真菌感染病原菌有助于早期诊断和治疗晚期肺癌患者深部真菌感染。本研究在细胞生物学水平对肺 癌患者深部真菌感染的主要病原菌进行研究,以探讨肺癌患者深部真菌感染发生发展过程中病原菌的变化 及其与发病的关系。方法 采用流式细胞技术(FCM)对肺癌患者深部真菌感染的主要病原菌———白念珠菌 和对照组白念珠菌细胞总DNA含量、增殖指数(PI)及细胞周期进行测量。结果 肺癌组与对照组稳定生长 阶段的白念珠菌细胞大多数处于G0/G1期。两组G0/G1、G2/M期的细胞构成比和细胞总DNA含量均无显 著性差异;肺癌组S期细胞构成比和增殖指数均显著高于对照组(P=0.040,P=0.038)。结论 肺癌患者深 部真菌感染主要病原菌———白念珠菌细胞DNA合成期比例增加,增殖活性增强,发生了致病力的变化。

Background and objective Deep fungal infection is one of the important complications and causes of death in late stage patients with lung cancer, to explore and analyze the pathogen of deep fungal infection is helpful to early diagnose and treat deep fungal infection in patients with lung cancer. The aim of this study is to investigate the changes and pathogenicity of the main pathogen of deep fungal infection in patients with lung cancer. Methods Total DNA per cell, proliferation index (PI) and cell cycle of the main pathogen of deep fungal infection were analyzed in patients with lung cancer by FCM. Results Histograms of stationary-growth-phase Candida albicans isolates demonstrated that the majority of population of the fungus were at the G0/G1 phase of cell cycle. There was no significant difference in cell proportion of G0/G1, G2/M phases and the total DNA content per cell of strains between the lung cancer and normal control groups. The cell proportion of S phase and PI in lung cancer group were remarkably higher than those in control group (P=0.040, P=0.038). Conclusion The cell proportion at DNA composition phase of the main pathogen--Candida albicans significantly increases, the proliferation activity gets strengthened and the pathogenicity changes in deep fungal infection of patients with lung cancer.