碱性成纤维细胞生长因子复合部分脱蛋白骨增强兔股骨头骨缺损修复作用的研究

STUDY ON THE EFFECT OF COMPOSITE OF BASIC FIBROBLAST GROWTH FACTOR AND PARTIALLY DEPROTEINIZED BONE ON THE REPAIR OF FEMORAL HEAD DEFECTS

目的 探讨碱性成纤维细胞生长因子 (basic fibroblast growth factor,b FGF)复合部分脱蛋白骨(partially deproteinised bone,PDPB)对股骨头骨缺损的修复作用。 方法 成年健康新西兰白兔 2 4只 4 8髋 ,随机分为3组 (n=16 ) ,切开关节囊 ,在头颈交界处开窗 ,制备骨缺损模型。 A组植入 b FGF/ PDPB,B组植入 PDPB,C组为空白对照 ,于术后 2、4和 8周分别处死动物 ,制备血管墨汁灌注标本 ,样本行 X线、组织学观察和图像分析。 结果 组织学观察 A组于术后 8周股骨头骨缺损完全修复 ,B组 8周时修复区移植材料未被完全爬行替代 ,C组仍有缺损残存。墨汁灌注显示 ,术后 2周时 A组修复区新生血管丰富 ,8周时与 B组无明显差异 ,C组各时相点血管稀少。术后 8周 X线片评价 ,A组 3侧优 ,2侧良 ,1侧可 ,优良率 80 % ;B组 1侧优 ,2侧良 ,2侧可 ,1侧差 ,优良率 5 0 % ;C组 1侧可 ,5侧差。术后 8周修复组织骨小梁体积分数 A组优于 B组 ,具有统计学意义 (P<0 .0 5 ) ,A和 B组都优于 C组 (P<0 .0 5 )。 结论 b FGF与 PDPB复合有效促进了兔股骨头骨缺损的修复。

Objective To evaluate the effect of composite (bFGF/PDPB) of basic fibroblast growth factor(bFGF) and partially deproteinized bone (PDPB) on the repair of femoral head defect. Methods Forty eight femoral heads with defect derived from 24 New Zealand rabbits were divided into 3 groups at random, which were implanted with bFGF/PDPB(group A), PDPB(group B) and nothing(group C) respectively. The rabbits were sacrificed at 2,4,and 8 weeks after operation, and then the femoral heads were obtained. The specimens injected with Chinese ink were created. Then X ray examination, histopathological and morphological examination of blood vessel, and image analysis were made. Results The bone defects healed completely 8 weeks after operation in group A. The implants in the repaired tissue were not substituted completely in group B. The bone defects did not heal completely in group C. Two weeks after operation, affluent newly formed vessels were seen in repaired areas in group A. No significant difference between group A and group B was observed 8 weeks after operation. In group C, newly formed vessels were scarce 2, 4, and 8 weeks after operation. There were 3 sides rated excellent, 2 good and 1 fair in group A; 1 excellent, 2 good, 2 fair and 1 poor in group B; and 1 fair and 5 poor in group C according to the X ray evaluation 8 weeks after operation. Eight weeks after operation, the volume fraction of bone trabecula in repaired tissue was higher in group A than that in group B ( P <0.05), and the fraction in group C was the lowest among the 3 groups ( P <0.05). Conclusion The composite of bFGF and PDPB can effectively promote the repair of femoral head defect of rabbit.