异体软骨细胞复合透明质酸苄基酯凝胶修复关节软骨缺损

Repair of articular cartilage defect with homogeneous chondrocytes combined with hyaluronic acid benzyl ester

目的 :研究采用同种异体软骨细胞复合透明质酸苄基酯凝胶修复关节软骨缺损的效果。方法 :将体外培养的同种异体第二代软骨细胞与透明质酸苄基酯凝胶复合注入兔关节软骨缺损区作为实验组 ,以软骨细胞悬液组和不作任何处理组作为对照 ,于第 4、8、1 2周取材 ,进行大体、组织学、电镜检查 ,观察其修复效果。结果 :实验组术后 1 2周时 ,缺损区可见新生透明样软骨组织 ,表面光滑 ,与周围软骨界面难以辨认。软骨细胞悬液组和空白对照组组均未见明显修复。结论 :同种异体软骨细胞复合透明质酸苄基酯凝胶修复关节软骨缺损效果明显 ,将透明质酸苄基酯凝胶作为移植载体是可行的。

Objective:To investigate the result of repairing the cartilage defect with homogeneous chondrocytes combined with hy aluronic acid benzyl ester. Methods:Homogeneous cartilage chondrocytes of New Zealand rabbits were harvested and cultured in vitro. The lateral condyle defect s were made in 30 rabbits. In the experimental group, the defects were repaired by homogeneous chondrocytes combined with hyaluronic acid benzyl ester; in the c ontrol group, the defects were repaired by cartilage chondrocytes only or were l eft un-repaired. The animals were sacrificed in the 4th,8th and 12th weeks afte r operation respectively and the macroscopic, histological, electron microscopic evaluations were performed. Results:In the experimental group, the cartilage d efects were repaired by the hyaline cartilage-like tissue. 12 weeks after of op eration, the defects were completely filled with mature cartilage tissue, which integrated smoothly with articular cartilage. In the control group, only a small amount fibrous tissues were seen on the surface of defects. Conclusion:The art icular cartilage defects of rabbits are repaired significantly with homograft of chondrocytes seeded onto hyaluronic acid benzyl ester, which is a promising way for the treatment of cartilage defects.