重组可溶性补体受体I型对大鼠急性脊髓损伤后神经功能的影响

Effect of recombinants CR1 on neural function following acute spinal cord injury of rats

目的 :探讨重组可溶性补体受体 I型 ( s CR1 )对大鼠急性脊髓损伤后神经功能的影响。方法 :采用改良 Allen重物打击法制成 SD大鼠脊髓急性损伤模型 ,观察 s CR1组与生理盐水 ( NS)组在伤后 1、3、7、1 4、2 1 d时间点损伤组织 C3c、C9、CD5 9阳性表达的部位及时程 ,采用斜板实验及 BBB评分法评定大鼠下肢神经功能。结果 :s CR1组在伤后各个时间点C3c、C9阳性表达均明显轻于 NS组 ,有非常显著性差异 ;s CR1组在伤后 1、3d时间点 CD5 9的表达明显轻于 NS组 ,有非常显著性差异 ,伤后 7d两组间有显著性差异 ,伤后 1 4、2 1 d两组间无显著性差异 ;s CR1组在伤后 7、1 4、2 1 d时间点大鼠下肢神经功能明显优于 NS组 ;结论 :重组可溶性补体受体 I型可通过抑制补体系统激活机制对急性脊髓损伤后的神经功能发挥显著性的保护作用。

Objective: To determine the effect o f recombinant soluble complement receptor type I (sCR1) on neural function follo wing acute spinal cord injury of rats. Methods: SD rat models were made by modif ied Allen's assay for acute spinal cord injury. C3c, C9, CD59 local expression s ites and time course in injury tissue at 1, 3, 7,14 and 21d after injury in sCR1 group and normal saline (NS) group were observed .Motor neural function of rat lower extremities in the two groups were evaluated by the tiltboard experiment a nd BBB score. Results: C3c and C9 positive expression in sCR1 group at each time point after injury was obviously less than that in NS group with highly signifi cant difference (P <0.01); CD59 positive expression in sCR1 group was obvio usly less than that in NS group with highly significant difference (P<0.01) at 1d and 3d, with significant difference (P<0.05) at 7d after injury, wit h no significant difference at 14d and 21d after injury. Rat neural function in sCR1 group at 7,14 and 21d was obviously better than that in NS group. Conclusi ons: Recombinant soluble complement receptor type I (sCR1) has significant prote ctive effects on neural function following acute spinal cord injury of rats by i nhibiting the activation of the complement system.