不同胃粘膜病变HP感染后端粒酶活性的表达

The association of telomerase activity erpression with Hp infection and gastric cancer

目的 :探讨端粒酶活性表达、HP感染和胃癌发生的相关性。方法 :对 1 1 6例活检标本及 42例手术标本采用快速尿素酶法及组织 Giemsa染色进行 HP检测 ,同时留取标本采用端粒重复序列扩增 -银染法进行端粒酶活性的检测。结果 :HP阳性率在正常胃粘膜、慢性萎缩性胃炎、肠上皮化生及胃腺癌中逐渐升高 ,阳性率分别为 35 .7%、5 0 %、5 0 %、62 .5 %、68.6% ,胃癌组与正常胃粘膜组差异具有显著性 ,端酶活性在正常胃粘膜中阴性 ,在胃癌中为 88.6% ,明显高于慢性萎缩性胃炎 ( 30 .6% )、肠上皮化生 ( 37.5 % )及不典型增生 ( 37.5 % ) ,差异具有显著性 ,在胃癌中端粒酶的活性与有无淋巴结转移有关 ,且 HP阳性胃癌组端粒酶阳性率 ( 97.9% )明显高于 HP阴性组 ( 67.8% ) ,差异具有显著性。结论 :1端粒酶活性表达出现在慢性萎缩性胃炎、肠上皮化生及不典型增生癌前病变中及高表达于胃癌中 ,提示其与胃癌的发生发展有关。 2端粒酶的激活可能与 HP感染有关。

Objective:To explore the asso ciation of telomerase activity expression with HP infection and gastric cancer.Methods: HP was examined using URT and hist ory Giemse dye in 116 cases livecheck specimens and 42 cases surgerical speci mens; simultaneously telomerase activity was tested by Telomeric repeat amplifi cation protocol(TRAP) silver dye test in all samples.Results:Positive rate of HP gradually increased in normal gastric mucous, chronic atrophy gastritis, inte s tines epithelium metaplasia, gastric adenocarcinoma,(35.7% , 50%, 62.5%, 68.6% respectivelly). Compared with the group of normal gastric m ucous, the positive rate in the group of gastric cancer increased significantl y. The telomerase activity in normal gastric mucous is negative,and 88.6% in ga stic cancer,which was apparently higher than in chronic atrophy gastritis(30.6%) , intesti ne epithelium metaplasia (37.5%), untypical hyperplasia(37.5%). The difference i s significant. The telomerase activity in gastric cancer is related to lymph no de metastasis, and the telomerase positive rae in gastric cancer with HP(+) was apparently higher than in that with HP(-), the difference has significance.co n clusions: 1. The expression of telomerase activity in chronic atrophy gastritis, intestine epithelium metaplasia, untypical hyperplasia and higher expression i n gastric cancer indicales that telomerase is associated with occurance and development of gastric cancer. Telomerase will probably become ideal marker of gastric cancer. 2. HP infection is likely related to the activitation of telom erase.