81例不同疾病患儿人细小病毒B19特异性抗体检测

Detection of specific antibodies to human parvovirus B19 in 81 children with various diseases

目的 调查细小病毒B19(humanparvovirusB19,HPVB19)在新疆地区不同疾病患儿中的感染情 况。方法 用ELISA法对2001年8月至2002年12月该院儿科81例住院患儿和来自儿保体检门诊16例健康儿 童(对照组)的血清标本进行了B19 IgM和B19 IgG检测。结果 B19 IgM阳性检出率在特发性血小板减少性紫癜 和再生障碍性贫血最高,分别为44.4%(4/9)和41.2%(7/17)。与对照组相比较差异有显著性(P<0.05),其它 疾病组与对照组相比差异无显著性(P>0.05)。而B19 IgG的检测结果,疾病组与对照组相比差异无显著性 (P>0.05)。结论 新疆地区特发性血小板减少性紫癜和再生障碍性贫血患儿中有较高的HBVB19感染率。特 发性血小板减少性紫癜和再生障碍性贫血与B19感染关系较为密切。

Objective To investigate the situation of human parvovirus B19 infection in children with various diseases in Xinjiang Province,PRC.Methods A total of 81 hospitalized children [18 cases of Henoch-Schoenlein purpura, 17 aplastic anemia (AA), 16 myocarditis, 9 idiopathic thrombocytopenic purpura (ITP), 8 acute lymphocytic leukemia, 8 respiratory infection and 5 nervous system diseases] in the First Affiliated Hospital of Xinjiang Medical University between August 2001 and December 2002 were involved in this study. Enzyme linked immunosorbent assay (ELISA) was employed to detect serum Bl9-IgM and B19-IgG. Serum samples from 16 healthy children were used as the Control group. Results Seven (41.2%) of 17 children with AA and 4 (44.4%) of 9 children with ITP were positive for B19-IgM. The positive rate for B19-IgM of children with either ITP or AA was significantly higher than that of the Control group ( 6.25%, 1/16) (both P<0.05).There were no significant differences in the positive rate for B19-IgM between the rest patients and the Control group.The positive rate for Bl9-lgG in all 81 patients was not different from the Control group.Conclusions The B19 infection rate was higher in children with ITP or AA in Xinjiang region. It is suggested that human parvovirus Bl9 may be closely related to ITP and AA.