菝葜化学成分研究

Studies on Chemical Constituents of Smilax china

目的 :研究菝葜根茎乙醇提取物中的化学成分。方法 :采用硅胶柱层析、分配层析、制备薄层层析等分离方法从乙醇提取物的乙酸乙酯部位分得七个单体化合物 ,并应用化学方法和现代波谱分析方法鉴定其结构。结果 :化合物Ⅰ鉴定为 5 O β D 葡萄糖 二氢山柰酚 (dihydrokaempferol 5 O β D glucoside) ,化合物Ⅱ鉴定为 β 谷甾醇(β sitosterol) ,化合物Ⅲ鉴定为胡萝卜苷 (Daucosterol)。其余化合物的结构鉴定尚在进行中。结论 :化合物Ⅰ为首次报道的新化合物。

Objective:To study the chemical constituents of the 95% ethanol extract from the rhizome of Smilax china Method:Seven compounds were isolated from ethyl acetate fraction of 95% ethanol extract by using repeated silica column chromatography,partition chromatography,preparing TLC,and other methods Structure of three compounds of them was identified based on chemical methods and spectroscopic methods Results:The structure of them were established as dihydrokaempferol 5 O β D glucoside(Ⅰ),β sitosterol(Ⅱ),daucosterol(Ⅲ) Conclusion:Compound Ⅰ was a novel compound which was first found from natural plants