松萝酸钠促皮肤创伤愈合的实验研究

The Study on Skin Wound Healing Promoting Action of Sodium Usnic Acid

目的:研究松萝酸钠对成纤维细胞增殖及促皮肤创伤愈合的影响.方法:培养L929成纤维细胞,体外加入不同浓度的松萝酸钠,MTT法观察其对成纤维细胞增殖的影响.整体观察松萝酸钠对大鼠实验性皮肤损伤的修复作用.结果:松萝酸钠浓度为0.5～8 μg/ml对L929成纤维细胞的增殖没有影响.整体实验表明,松萝酸钠外涂对实验性大鼠皮肤损伤有加速修复作用.松萝酸钠对成纤维细胞增殖无促进作用.结论:松萝酸钠具有促伤口愈合作用,其促修复作用不是通过促进成纤维细胞增殖而加速皮肤创伤愈合.

Objective: To study the effects of sodium usnic acid on proliferation of fibroblasts and skin wound healing.Methods: L929 fibroblasts were incubated in culture medium containing sodium usnic acid, the proliferation of fibroblasts were measured by MTT.Skin wound healing promoting action of sodium usnic acid was determined in rats.Results: Sodium usnic acid does not showed proliferation-promoting activity for L929 fibroblasts, sodium usnic acid by external application accelerated healing of rat backskin wound.The wound-healing rate of sodium usnic acid was higher than that of the control group at the 2th～12th day after treatment.Conclusion: Sodium usnic acid can accelerate skin wound healing, the effects was not by stimulating proliferation of fibroblasts.