P33^(ING1b)蛋白表达与胃癌分化程度的关系

Relationship between the p33^(ING1b) Protein Expression and Differentiated Degree in Human Gastric Carcinoma

目的 研究ING1基因表达产物P3 3 ING1b蛋白在胃癌中的表达 ,分析ING1基因表达与胃癌分化程度的关系。方法 采用S -P免疫组化法 ,检测P3 3 ING1b蛋白在不同分化程度胃癌及正常胃粘膜组织中的表达状况。结果 P3 3 ING1b蛋白在胃癌中的阳性表达率为 :高分化胃癌 75 % (15 /2 0 ) ;中分化胃癌 5 5 % (11/2 0 ) ;低分化胃癌 15 % (3 /2 0 ) ;正常胃粘膜组织P3 3 ING1b蛋白均为阳性表达(10 0 % ,60 /60 )。胃癌组织中P3 3 ING1b蛋白阳性表达率低于正常胃粘膜 ,差异有显著性意义 (P <0 0 5 ) ;P3 3 ING1b蛋白在胃癌中的表达率随着其分化程度的降低而减少 (P <0 0 5 )。结论 胃癌组织中存在P3 3 ING1b蛋白表达缺失 ,其表达缺失率与胃癌分化程度有关。

Objective To investigate the relationship between the p33 ING1b protein expression and differentiated degree in human gastric carcinoma. Methods p33 ING1b protein expression was detected by S-P immunohistochemical method in gastric carcinoma and normal gastric mucous tissues. Results The positive rates of p33 ING1b protein expression were 75% (15/20) in well-differentiated, 55% (11/20) in moderate-differentiated and 15% (3/20) in poor-differentiated gastric carcinoma, respectively. The positive rate of p33 ING1b protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa (100%, 60/60) (P<0.05). The p33 ING1b expression was positively related with differentiated degree of gastric carcinoma (P<0.05). Conclusion There was a loss expression of p33 ING1b protein in gastric carcinoma. The loss expression of p33 ING1b protein was related to differentiated degree of gastric carcinoma.