胶原-聚羟基乙酸与骨髓间质干细胞的细胞相容性研究

A Study on the Biocompatibility between Collagen-Polyglicolic Acid and Bone Marrow Mesenchymal Stem Cells

目的 研究胶原 -聚羟基乙酸与骨髓间质干细胞的细胞相容性 ,为构建组织工程化肌腱提供实验基础。方法 分离、培养并鉴定骨髓间质干细胞 ,将骨髓间质干细胞置入含胶原 -聚羟基乙酸的DMEM培基中培养 ,设为实验组 ;将骨髓间质干细胞置入纯DMEM培基中培养 ,设为对照组。比较两组细胞的活性和生长情况 ,并对实验组进行超微结构观察。结果 骨髓间质干细胞接种于胶原 -聚羟基乙酸中后可适应材料生长 ,混合培养后 2周生长良好 ,始终保持 89%以上的细胞活力 ,与对照组比较无显著差别 (P >0 0 5 ) ;在 2周的培养过程中实验组细胞数未发生明显改变 ,而对照组从第 4d开始即发生增殖。透射电镜示实验组细胞培养 14d后仍保持旺盛的分泌功能。结论 骨髓间质干细胞与胶原 -聚羟基乙酸的细胞相容性好。以骨髓间质干细胞为种子细胞 ,以胶原 -聚羟基乙酸为支架可在体外预构组织工程化肌腱。

Objective To investigate the biocompatibility between collagen- polyglycolic acid (PGA) and bone marrow mesenchymal stem cells (MSCs) in vitro to provide some experimental basis for further study in tendon tissue engineering. Methods MSCs were isolated, cultured and characterized. In the experimental group the MSCs were cultured in DMEM containing type-I collagen and PGA suture, and in the control group the MSCs were cultured in DMEM. The cell growth was compared between the two groups, and the cell ultramicroscopic structure of experimental group was observed. Results MSCs grew well in the collagen-PGA scaffold, and 2 weeks after incubation they still kept secretion potential and more than cell 89% vitality, which were not significantly different compared with the control group. There is no statistical difference in the MSCs count in the experimental group during 2 weeks culture, while in the control group MSCs began to proliferate at the 4th day after culture. Conclusion Collagen-PGA has a good biocompatibility with mesenchymal stem cells. It is possible to fabricate a tissue-engineered tendon in vitro using mesenchymal stem cells as seed cells and collagen-PGA as scaffold.