白细胞介素-13对小鼠肺组织粘蛋白基因Muc5ac及EGFR表达的作用

The Effects of Interleukin 13 on Mucin Gene Muc5ac and EGFR Expression in Mouse Lungs

目的 研究白细胞介素 -13 (IL -13 )对小鼠气道粘蛋白基因Muc5ac和表皮生长因子受体 (EGFR)表达的作用。方法 2 4只雄性BALB/c小鼠随机分为对照组和不同剂量IL -13 (包括 5 0ng、10 0ng和 2 5 0ng)处理组 ,每组 6只 ,分别用RT -PCR和免疫组化法检测Muc5acmRNA、Muc5ac和EGFR蛋白的表达。结果 Muc5ac和EGFR蛋白阳性表达细胞均主要位于气道上皮 ,细胞胞浆呈棕黄色。与对照组相比 ,IL -13可以剂量依赖性地促进气道Muc5acmRNA、Muc5ac和EGFR蛋白的表达 (P均 <0 0 1)。结论 IL-13是引起气道粘液过度分泌的重要细胞因子 ,它可能通过活化EGFR导致了上述病变。

Objective To study the effect of interleukin 13(IL-13)on mucin gene Muc5ac and EGFR expression in mouse lungs. Methods Twenty-four male BALB/c mice were randomly divided into control group and different dose of IL-13 (50ng, 100ng and 250ng) groups, six mice per group. Muc5ac mRNA, and Muc5ac and EGFR protein expressions were detected by RT-PCR and immunohistochemical method, respectively. Results Both Muc5ac and EGFR positive products were located in the cytoplasm of airway epithelial cells. Compared with control group, IL-13 could increase Muc5ac mRNA, and Muc5ac and EGFR protein expressions in dose-dependent manner(all P<0.01). Conclusion IL-13 is a critical cytokine inducing airway mucus overproduction, and may causes the above pathological changes through activation of EGFR.