摘要
动物细胞培养是生物技术的一个重要领域 ,因为培养的细胞可以用来分离纯化病毒、生产疫苗和药物。本实验在日本对虾肝胰腺细胞和血细胞原代培养的基础上 ,利用RNA DNA值进行不同pH值培养基的比较 ,认为对于血细胞pH 7 2为最适值 ,而对于肝胰腺细胞pH 7 6比较合适。同时利用光镜及电镜做了一些形态和结构上的观察。
Culture of animal cells is an important area of biotechnology because the cells can be used for virus isolation, vaccine and medicine production. In the present study, RNA/DNA ratio was used to evaluate three kinds of media of different pH values. The results showed that pH 7.2 was the optimized condition for hemocyte culture while pH 7.6 was a proper condition for hepatopancreatic cell culture in Penaeus japonicus. The morphology, structure and ultrastructure of cells were also examined.
出处
《动物学杂志》
CAS
CSCD
北大核心
2005年第1期88-91,共4页
Chinese Journal of Zoology
基金
河北省动物学重点学科基金
河北大学自然科学青年基金项目 (No.Q2 0 0 4 0 7)
关键词
日本对虾
细胞培养
肝胰腺
血细胞
Penaeus japonicus
Cell culture
Hepatopancreas
Hemocyte