不同pH值条件下日本对虾肝胰腺及血的细胞培养

Culture of Hepatopancreatic Cells and Hemocytes under Different pH Values in Penaeus japonicus

动物细胞培养是生物技术的一个重要领域 ,因为培养的细胞可以用来分离纯化病毒、生产疫苗和药物。本实验在日本对虾肝胰腺细胞和血细胞原代培养的基础上 ,利用RNA DNA值进行不同pH值培养基的比较 ,认为对于血细胞pH 7 2为最适值 ,而对于肝胰腺细胞pH 7 6比较合适。同时利用光镜及电镜做了一些形态和结构上的观察。

Culture of animal cells is an important area of biotechnology because the cells can be used for virus isolation, vaccine and medicine production. In the present study, RNA/DNA ratio was used to evaluate three kinds of media of different pH values. The results showed that pH 7.2 was the optimized condition for hemocyte culture while pH 7.6 was a proper condition for hepatopancreatic cell culture in Penaeus japonicus. The morphology, structure and ultrastructure of cells were also examined.