干扰素对日本血吸虫虫卵肉芽肿小鼠肺模型的免疫调节作用

Effects of interferon-α on immunoregulation of mice with schistosoma japonicum eggs granuloma in lung model

研究目的探讨干扰素在虫卵肉芽肿病理过程中的作用及血吸虫病的发病机制。方法用纯系NH小鼠感染日本血吸虫虫卵建立小鼠肺部虫卵肉芽肿模型。观察干扰素α对肉芽肿及小鼠免疫反应的影响感染小鼠后分别于第4、8、16、24、32天解剖动物，检测其血清抗体滴度、胸腺T细胞百分比和肉芽肿最大横切面面积。结果干扰素组小鼠抗体滴度、胸腺T细胞百分比在不同时程呈动态变化趋势，其肉芽肿面积在第8天明显增大，以后逐渐缩小。结论干扰素对血吸虫虫卵肉芽肿病变过程具有免疫调节作用。

Objective To investigate the effect of Interferon -α on the pathological process of mice with the eggs granuloma and the pathogenesis of schistosomiasis.Methods The model of pulmonary granuioma was established by infecting homozygote NIH mice With Schistosoma japonicum eggs The effects of Interferon-α on the immuno-regulation of the mice were observed.After being infected mice were dissected in the 4th, 8th, 16th, 24th and 32th day respectively to test the antibody titer of the serum.the T-cell percentage in thymus and the size of the granuloma area on the biggest transverse section.Results The changes of serum antibody titer and the T-cell percentage in interferon group were dynamic during various periods, and the granuloma size was increased in the 8th day and then attenuated gradualfy.Conclusion All the resuits indicate the different effects of Interferon - α on pathological process of this model during various periods and prompt it's immuno-regulation effects.