海藻基根硬毛藻的化学成分研究

Chemical constituents from marine alga Chaetomorpha basiretorsa

目的对绿藻基根硬毛藻Chaetomorphabasiretorsa进行化学成分研究,并对单体化合物进行细胞毒活性筛选。方法利用正相和反相色谱、SephadexLH20色谱以及反相HPLC等手段进行分离纯化,应用MS,IR,NMR和Xray单晶衍射等方法鉴定结构,通过MTT法对单体化合物进行细胞毒活性筛选。结果从基根硬毛藻中分离并鉴定了9个化合物,分别为N苯基2萘胺(Ⅰ),邻苯二甲酸二丁酯(Ⅱ),邻苯二甲酸二异丁酯(Ⅲ),苯乙二醇(Ⅳ),邻羟基苯甲醛(Ⅴ),二甘醇单苯甲酸酯(Ⅵ),尿嘧啶(Ⅶ),胸腺嘧啶(Ⅷ),胸腺嘧啶脱氧核苷(Ⅸ)。化合物Ⅰ对KB细胞具有一定的选择性毒性(IC50,10.15μg·mL-1);尿嘧啶(Ⅶ)对KB细胞(IC50,3.79μg·mL-1)和MCF7细胞(IC50,3.24μg·mL-1)也具有选择性毒性。结论所有化合物均为首次从本属海藻中分离得到,其中N苯基2萘胺(Ⅰ)和二甘醇单苯甲酸酯(Ⅳ)为首次从海洋生物中分离得到;N苯基2萘胺(Ⅰ)和尿嘧啶(Ⅶ)具有一定选择性细胞毒活性。

Objective: To investigate the chemical constituents of marine alga Chaetomorpha basiretorsa. Method: Compounds were isolated by normal phase silica gel and Sephadex LH-20 gel colum chromatography, reverse phase MPLC, reverse phase HPLC and recrystallization. Their structures were elucidated by spectroscopic methods including MS, IR, NMR, and X-ray crystalography. Cytotoxicity of the compounds were screened by using standard MTT method. Result: Nine compounds were isolated from C. basiretorsa and their structures were identified as N-phenyl-2-naphthalenamine(Ⅰ), dibutyl phthalate(Ⅱ), diisobutyl phthalate(Ⅲ), 1-phenyl-ethane-1, 2-diol(Ⅳ), 2-hydroxy-benzaldehyde(Ⅴ), diethyleneglycol monobenzoate(Ⅵ), uracil(Ⅶ), thymine(Ⅷ) and thymidine(Ⅸ). Conclusion: All these compounds were obtained from this genus for the first time, N-phenyl-2-naphthalenamine and diethyleneglycol monobenzoate were first reported from the marine organisms. Compound Ⅰ and Ⅶ showed moderate activity against KB cell(IC_~50 10.15 μg.mL^-1 for Ⅰ and 3.79 μg.mL^-1 for Ⅶ) and MCF-7 cell(IC_~50 3.24 μg.mL^-1 for Ⅶ). [