健胎液对胚泡着床障碍小鼠雌、孕激素及其受体的影响

Effects of Jiantai liquid on the expression of estrogen/progesterone receptors in embryo implantation dysfunction mice endometrium

目的探讨健胎液促进胚泡着床障碍小鼠子宫内膜发育的机制。方法采用米非司酮诱导的小鼠胚泡着床障碍模型,予健胎液进行干预,于妊娠第8天处死小鼠,留取血清及子宫标本,采用放免法检测血清和子宫局部雌二醇(E2),孕酮(P)含量,采用免疫组化技术检测子宫雌,孕激素受体(ER,PR)表达。结果各组血清和子宫组织E2,P含量无显著差异(P>0.05);中药组子宫内膜腺体、间质ER,PR表达范围和强度均较模型组显著增高(P<0.05),与正常组无差异(P>0.05)。结论健胎液通过促进胚泡着床障碍小鼠子宫内膜腺体、间质的ER,PR表达,改善子宫内膜的发育,利于胚泡着床。

Objective: To explore the mechanism of Jiantai liquid on the endometrium development of embryo implantation dysfunction mice. Method: The model of embryo implantation dysfunction mice was induced by mifepristone and treated by Jiantai liquid. All animals were sacrificed on day 8 of pregnancy. Estradiol and progesterone concentrations in serum and endometrium tissue homogenates were measured by radioimmunoassay method,the endometrial expressions of estrogen receptor (ER)and progesterone receptor (PR)assessed by immunnohistochemical SP method. Result: There were no significantly differences in the estradiol and progesterone concentrations in serum and uterus tissue homogenates among three groups(P>0.05). Absorbency and area rate of ER, PR in model group’s gland and stroma were higher than those in model group(P<0.05), which was similar with the control group(P>0.05). Conclusion: Jiantai liquid increase the implantation rate and improve the endometrial development by increasing the expressions of ER, PR in endometrium of embryo implantation dysfunction mice. [