血红素氧合酶-1与大鼠肾缺血再灌注损伤关系的研究

The relationship between heme oxygenase-1with renal ischemia reperfusion injury in rat

目的 :探讨血红素氧合酶 -1(hemeoxygenase -1,HO -1)与大鼠急性肾缺血再灌注损伤 (IRI)的关系。方法 :建立大鼠肾缺血再灌注损伤模型 ,分别检测48只大鼠缺血前及再灌注24h后肾IRI组织中HO -1的活性与含量 ,以及血尿素氮 (BUN)、肌酐 (Cr)、超氧化物岐化酶 (SOD)和丙二醛 (MDA)的水平。结果 :HO -1的活性与SOD呈正相关 (r=0.663 ,P<0.001),与BUN、Cr、MDA呈负相关 (r= -0.723 ,P<0.001 ;r= -0.630 ,P<0.005 ;r= -0.629,P<0.01) ;HO -1的含量与SOD呈正相关 (r=0.570,P<0.001),与BUN、Cr、MDA呈负相关 (r= -0.646,P<0.005;r= -0.577 ,P<0.001 ;r= -0.530 ,P<0.01)。结论 :HO -1与IRI关系密切 ,可通过清除氧自由基 (OFR)而减轻IRI及保护肾功能。

Objective:To investigate the relation between Heme Oxygenase-1(HO-1)and kidney normothermic ischemic reperfusion injury(IRI)in rat.Methods:Rat kidney normotherˉmic ischemic reperfusion model was established.Twenty-four hours after reperfusion,content and activity of HO-1of renal tissue,Superoxide dismutase(SOD),malondialdehyde(MDA),blood urea nitrogen(BUN)and serum creatinine(Cr)were examined.Results:Statistical analyˉsis revealed a positive correlation between activity of HO-1and SOD( r =0.663,P<0.001)and a negative correlation between HO-1and BUN or Cr or MDA( r =-0.723,P<0.001; r =-0.630,P<0.005; r =-0.629,P<0.01),and a positive correlation between content of HO-1and SOD( r =0.570,P<0.001)and a negative correlation between HO-1and BUN or Cr or MDA( r =-0.646,P<0.005; r =-0.577,P<0.001; r =-0.530,P<0.01).Concluˉsion:These findings indicate that HO-1could attenuate the subsequent renal IRI and protect renal function possibly by eliminating the toxic oxygen free radicals(OFR).