哈密瓜花粉离体萌发特性的研究

Study on in Vitro Sprouting Characteristic of Pollen in Hami-melon

通过哈密瓜花粉在固体培养基上进行离体培养,对哈密瓜品种金皇后的离体萌发特性进行研究。结果表明,培养基中硼酸的不同浓度对哈密瓜花粉离体萌发具有明显的影响,适宜的硼酸浓度为20mg/L;在不同的温度条件下,哈密瓜花粉的萌发率不同,25℃时哈密瓜花粉的萌发率达到了最大值,花粉萌发的适宜温度范围为10～35℃,随着温度的升高,花粉管的长度也相应的增加;另外,不同的贮藏时间和贮藏温度对哈密瓜花粉的萌发率也有一定的影响,一般在低温条件下贮藏花粉,可以较长时间保持花粉较高的萌发率。

By in vitro cultured the Hami-melon pollen on the solid media,the result shows that the different concentrations of boric acid have obvious influence to the sprouting rate of Hami-melon pollen.The suitable concentration of boric acid is 20mg/L.Under different temperature conditions,the sprouting rate of the Hami-melon pollen is different.The sprouting rate is at about 25℃ maximum.The suitable temperature range of pollen sprouting is between 10～35℃,and with the rising of temperature,the length of pollen tube also increases correspondingly.In addition,in lower temperature,the pollen can be remained the higher sprouting rate for longer time.