何杰金氏病肿瘤细胞生物学特征研究

A Cellular Biological Study for Tumor Cells of Hodgkin's Disease

目的 对何杰金氏病肿瘤细胞进行形态学观察和免疫表型研究。材料方法 福尔马林固定,石蜡包埋的何杰金氏病组织12例(NSHL6例,MCHL5例,LDHL1例)进行HE染色和CD30、CD15、CD20、CD45RO、Ki-67抗原免疫组化在肿瘤细胞和背景成分中的表达情况。结果 (1)R-S细胞有各种变体形式。(2)各型HL具有不同的背景成分和组织学特征。(3)NSHL肿瘤细胞表达CD30(6/6)、CD15(1/6),不表达CD20(0/6)。MCHL肿瘤细胞表达CD30(5/5)、CD15(0/5),LDHL肿瘤细胞表达CD 30(1/1)。CD30阳性率明显高于CD15(0.01<P<0.025)。结论 (1)NSHL中纤维组织增生形成结节,瘤细胞以陷窝细胞为主;MCHL中诊断型R-S细胞及其它变体细胞数量较多;LDHL以淋巴细胞减少为其特征。均以表达CD30为其特征。(2)在何杰金氏病鉴别判断中一定要应用免疫组化。CD30、CD45RO、Ki-67、CD20一般为一线抗体。

Objective To investigate the histopathologieai and immunophenotypic characteristics of 12 cases of Classical Hodgkin's lymphoma. Materials and Methods Formalin fixed and paraffin embedded tissues from 12 case were studied by HE and immunostaining for CD20 , CD30 , CD5RO·CD15 , and Ki-67 antigens for tumor cells. Results (1) Hodgkin / Reed -Sternberg cells have a lot of variants. (2) Differential histological and immunophenotypic characteristics were found in these samples. (3) The NSHL cells showed CD30 (6/6) and CD15 (1/6) and not show CD20(0 / 6). The MCHL cells showed CD30(5 / 5)、CD15(0 / 5) ,The LDHL cells showed CD30 (1 /1). The rate of positive reaction is higher than CD15 (0. 01 < P < 0. 025). Conclusions (1) Each subtype of CHL has distinctive histopathological and immunophenotypic characteristics. Malignant cells mainly expresses CD30 , but rarely CD15 . (2) Special attentions should be paid to the application of immunohistochemistry for CD30 ,CD15 , CD20 and Ki-67 .