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RNA沉默—新型的植物病毒病害防治策略 被引量：8

RNA silencing—a new strategy for the control of virus diseases in plants.
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摘要 植物RNA沉默(RNAsilencing)是植物本身固有的一种抗病毒防御系统,是对病毒在复制过程中形成双链RNA(dsRNA)的特殊反应。RNA沉默介导的dsRNA干涉病毒侵染转基因抗病毒有其不足,几种体外生产dsRNA的抗病毒体系能弥补这一不足,它们与转基因表达病原RNA不同,但仍然依赖RNA沉默机制来获取病原抗性。综述了近年来发展起来的各种启动RNA沉默的dsRNA产生策略。 RNA silencing occurs in plants is conceived as a natural antiviral defense system that is activated as a response to double?stranded RNA (dsRNA) formed during virus replication.To compensate for the disadvantages on RNA silencing by using dsRNA to specifically interfere with virus infection in transgenic plants,several approaches of obtaining dsRNA used for virus?resistance have been developed which differ from strategies based on transgenic expression of RNAs but still rely on RNA silencing as a means to achieve pathogen?derived resistance (PDR).In this paper,the dsRNA produce strategies for RNA silencing,their applications for virus resistance,disadvantages and perspectives are reviewed.
作者 牛颜冰 郭失迷 宋艳波 雷万钧 申林炎
机构地区 山西农业大学
出处 《中国生态农业学报》 CAS CSCD 2005年第2期47-50,共4页 Chinese Journal of Eco-Agriculture
基金 山西农业大学优秀人才引进基金项目 山西农业大学博士启动基金项目
关键词 RNA沉默 双链RNA 抗病毒 RNA silencing Double-stranded RNA Virus resistance
分类号 S432.41 [农业科学—植物病理学] Q756 [生物学—分子生物学]
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参考文献20

  • 1牛颜冰,青玲,周雪平.RNA沉默机制及其抗病毒应用[J].中国生物工程杂志,2004,24(2):76-79. 被引量：25
  • 2牛颜冰,于翠,张凯,崔晓峰,陶小荣,周雪平.瞬时表达黄瓜花叶病毒部分复制酶基因和番茄花叶病毒移动蛋白基因的dsRNA能阻止相关病毒的侵染[J].农业生物技术学报,2004,12(4):484-485. 被引量：15
  • 3Hammond S.M.,Caudy A.A.,Hannon G.J.Post-transcriptional gene silencing by double-stranded RNA.Nat.Rev.Genet.,2001,2(2): 110～119.
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二级参考文献21

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二级引证文献22

中国生态农业学报
2005年 第2期

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