宽叶缬草对血管性痴呆小鼠学习记忆及海马区神经元病理学改变的影响

Effect of Valeriana Officinalis Var Latifolia Miq on Study and Memory Ability and Pathomorphology Change of Hippocampal Neuron in Vascular Dementia Model Mice

目的:观察宽叶缬草对血管性痴呆模型小鼠学习记忆及海马区神经元病理学改变的影响。方法:采用反复夹闭双侧颈总动脉结合腹腔注射硝普钠的方法复制小鼠拟血管性痴呆的模型。健康昆明小鼠54只,随机分为3组:假手术组、血管性痴呆模型组、宽叶缬草组。分别于术后7、15、30 d,跳台实验检测其痴呆程度;透射电镜、HE和Nissl染色对海马区神经元病理学改变进行观察。结果:宽叶缬草组跳台实验潜伏期明显短于模型组(P<0.01),受电击总时间少于模型组(P<0.01);宽叶缬草组海马CA1区神经元数量明显多于模型组(P<0.01);模型组可见神经元脱失、部分神经细胞核固缩并有胶质细胞增生等病理学改变。结论:宽叶缬草能明显减轻血管性痴呆小鼠海马CA1区神经元损伤,改善脑缺血引起的学习记忆障碍。

Aim:To observe the effect of Valeriana Officinalis Var Latifolia Miq(VOL)on study and memory ability and pathomorphology change of hippocampal region in vascular dementia model mice. Methods: Vascular dementia model in mice was established by clamping of the both common carotid artery repeatedly. Forty Kunming healthy mice were divided into 4 groups randomly,sham-operation group,model group and VOL group.On the 7th, 15th,30th after operation,grade of dementia was evaluated by Step-down avoidance test, pathomorphology change of hippocampal neuron was observed by electron microscope and hematoxylin-eosin( HE), Nissl staining. Results: Laten period in VOL group was shorter than that in model mice( P < 0.01). The quantities of neuron in VOL group in hippocampus CA1 were more than that in model group ( P < 0.01) .The loss of neuron,karyopyknosis in partial neurons and proliferation of glial cells in hippocampal CA1 were found in vascular dementia mice which relived markedly in VOL group. Conclusion: VOL can obviously improve pathomorphology change of hippocampal CA1 neuron and the ameliorate study and memory ability in vascular dementia mice.