人脑动静脉畸形血管平滑肌细胞的培养和鉴定

Culture and Identificaltion of Vascular Smooth Muscle Cell from Human Cerebral Arteriovenous Malformation

目的:建立一个稳定的人脑动静脉畸形血管平滑肌细胞的培养体系。方法:取手术切除的人脑动静脉畸形组织块以酶消化法接种于铺有胶原底层的培养瓶中进行原代及传代培养,并对其进行形态学观察、免疫组化染色及透射电镜等一系列细胞定性研究。结果:接种后6-8 d后可见少量细胞贴壁,所获培养细胞经免疫组化、透射电镜鉴定证实为血管平滑肌细胞,而且细胞纯度较高,细胞可以连续传代18代。结论:应用酶消化法可获得纯化的血管平滑肌细胞,细胞可以连续传代培养。培养动静脉畸形血管平滑肌细胞可应用于体外作进一步的深入研究。

Aim: To developed a procedure for the isolation and culture of vascular smooth muscle cells from human cerebral arteriovenous malformation. Methods: The surgical specimens were digested with collagenase type IV . The cell population then was placed on the dishes percoated with gelatin for primary culture. The cell line derived was then analyzed by cellular morphology, immunochemistry and electromicroscopy. Results: The first growing cell colonies appeared 6 to 8 days after initial seeding. The cell was confirmed to be VSMC by immunochemistry analysis and the morphological and ultracstructural characteristics were consistent with that of VSMC.The cells still grows well after 18 generations. Conclusion: A purified VSMC cell line can be developed by collagenase digest assay and the cells can be used as a cellular model in vitro.