枸杞多糖对动脉粥样硬化模型兔血脂、脂质过氧化、NO和C-反应蛋白的影响

Effect of lycium barbarum polysaccharide against atherosclerosis in rabbits

目的:探讨枸杞多糖对新西兰兔动脉粥样硬化的预防作用及其机制。方法:36只雄性新西兰兔随机分 为3组,每组12只。对照组喂普通饲料,模型组采用普通饲料加质量分数1.5%的胆固醇喂养,枸杞多糖(LBP)组 采用普通饲料加质量分数1.5%的胆固醇喂养,同时腹腔注射LBP(3.0mg/kg),10周后,测定各组空腹血清三酯酰 甘油(TG)、总胆固醇(TCh)、高密度脂蛋白胆固醇(HDL- C)、一氧化氮(NO)、丙二醛(MDA)、C -反应蛋白(CRP)含 量以及超氧化物歧化酶(SOD)活性,计算主动脉内膜粥样斑块面积并观察主动脉形态学变化。结果:模型组与对照 组比较,TG、TCh、NO、MDA、CRP升高(P<0.01),HDL- C/TCh、SOD活性下降(P<0.01),主动脉内膜粥样斑块面积 较大(P<0.01);LBP组与模型组比较,TG、NO、MDA、CRP下降(P<0.01或0.05),HDL -C/TCh、SOD活性升高 (P<0.01),主动脉内膜粥样斑块面积明显减少(P<0.05)。结论:枸杞多糖对动脉粥样硬化有明显的预防和治疗 作用,可能与其抗氧化、免疫调节、提高HDL- C/TCh有关。

Aim: To study the effect and mechanism of lycium barbarum polysaccharide(LBP) against atherosclerosis in rabbits. Methods: Thirty-six healthy New Zealand white rabbits were randomly divided into three groups.Control group was fed with normal food,model group was fed with 1.5% cholesterol,and LBP group, 1.5% cholesterol plus 3.0 mg/kg LBP celiac injection. After ten weeks,the concentrations of triglyceride(TG),total cholesterol,high density lipoprotein cholesterol(HDL-C),C reactive protein(CRP),nitric oxide(NO),MDA,and the activity of SOD of each group were measured. Results: Compared with the control group,concentrations of TG,TCh,NO,MDA,and CRP of model group increased significantly(P<0.01),and activity of SOD and HDL-C/TCh decreased(P<0.01). Compared with the model group,concentrations of TG,NO,MDA,and CRP of LBP group significantly lowered(P<0.01 or 0.05), while the activity of SOD and HDL-C/TCh increased(P<0.01). Conclusion: LBP can effectively increase the activity of SOD against oxidation,regulate immunity,and elevate the radio of HDL-C/TCh.LBP is a potential drug in treating and preventing atherosclerosis.