影响刺孢小克银汉霉菌菌体形态和γ-亚麻酸含量的因素

Factors Affecting Morphology of Cunninghamella echinulata and Production of γ-linolenic Acid

在获得1株高产γ-亚麻酸 (gamma linolenic acid,GLA)的刺孢小克银汉霉菌(Cunninghamella echinulata)的基础上,观察了影响其菌体形态和GLA的各个因素的影响,结果表明:起始pH、装液量、离子的类型和浓度、接种体类型等对刺孢小克银汉霉菌的形态影响很大.起始为pH 5,装液量(500 mL三角瓶)为50～100 mL以及适当的离子浓度有利于刺孢小克银汉霉菌体保持分散的形态,并且可以大幅度提高GLA的含量.利用纯孢子液接种始终造成刺孢小克银汉霉菌体形态成为球形,GLA含量较低,处于10%以下,而菌块接种形态成絮状,GLA含量较高,可达13%左右.

Factors affecting morphology of Cunninghamella echinulata and production of γ-linolenic acid were studied. The result showed that original pH, aeration, ions and inoculum types-spore or muycelia had great effects on morphology of C.echinulata .Original pH 5,media 50~100 mL per 500 mL flask and concentrations of ions were important for the mycelium deaggregation and increase of GLA production. The morphology of C. echinulata was always pellets when using spores as inocula. But the morphology and GLA content were much more stable when inoculated with mycelia.