缺血再灌注损伤心肌电镜细胞化学的定性与定量分析─三磷酸腺苷酶（一）

Qualitative and Quantitative Studies on Cytochemistry of Ischemia-Reperfusion Injury of Myocardium by Electronic Microscope.I.Adenosine Triphosphatase.

本研究选择心脏瓣膜病患者２０例，随机分为两组，每组１０例．在瓣膜置换术中分别采用冷晶体停跳液（Ｓｔ．Ｔｈｏｍａｓ液）间断灌注（低温组）和温血停跳液持续灌注（常温组）的心肌保护方法．缺血期末和再灌注３０分钟心肌三磷酸腺苷酶（ＡＴＰ酶）电镜细胞化学定性分析结果示两组各期心肌线粒体和粗面内质网ＡＴＰ酶染色均为阳性，计算机图像分析结果表明ＡＴＰ酶活性灰度值组内及组间比较均无显著差异．由于影响ＡＴＰ酶活性的因素较多，故不能特异地反映心肌功能和结构的损伤程度．

We selected 20 patients undergoing valvular surgical procedures and divided them into two groups with random in this study.Intermittent perfusion of cold crystalhd(St.Thomas Hospital fluid)(hypothermic group)and continuous administration of warm blood cardioplegia(normothermic group)were applied respectively. Samples of myocardium were taken both at the end of the ischemic peried and 30 minutes after reperfusion from each patient.Qualitative results of cytochemistry of adenosine triphosphatase (ATPase) of myocardial mitochondria and rough endoplasmic reticulum by electronic microscope all showed positive in each period of the two groups, And there were no significant differences in the gray level of ATPase activities within and between the two groups by computerizing the photographs,In conclusion, it illustrated that ATPase might not specifically reflect the functional or structural injury extent because of multiple factors concemed.