BN大鼠食物过敏动物模型的实验研究

Assessment of brown Norway rat as suitable animal model for investigation of food allergy

为了解挪威棕色大鼠 (BN)作为食物过敏动物模型的可行性。将 2 4只BN大鼠随机分为灭菌水组 (对照组 )、卵清蛋白组 (Ovalbumin ,OVA)、马铃薯酸性磷酸酶组 (Potatoacidphosphatase ,PAP)、鸡蛋清粗提蛋白质组 (hen’segg whiteprotein ,HEWP) ,每组 6只。对各组大鼠灌胃 ,1ml 只 ,OVA、PAP组蛋白质浓度为 1mg ml,HEWP组蛋白质浓度为 10 0mg ml,每天 1次 ,共 6周。检测血清中特异IgE抗体滴度 ,同时进行皮肤过敏反应试验 (PCA)。在第 2 8、4 2天 ,OVA、HEWP组BN大鼠 32倍稀释血清中特异IgE抗体均较对照组升高 ,并有统计学差异 ,第 14、2 8、4 2天的PAP组及第14天的OVA、HEWP组BN大鼠 32倍稀释血清中特异IgE抗体较对照组相比无统计学差异。BN大鼠对常见致敏食物蛋白质OVA和HEWP产生过敏反应 ,对无致敏史食物蛋白质PAP无过敏反应。BN大鼠模型可能是较为理想的食物过敏动物模型。

To develop an animal model that closely mimics human food allergy, 4 groups of BN rats were given one of the following substances by gavage dairly for 42 days: Ovalbumin(1mg in 1ml), Potato acid phosphatase (1 mg in 1 ml), hens egg white protein (10 mg in 1 ml) and sterile water(1 ml).The serum levers of specific IgE antibodies were measured on the 14th, 28th and 42nd day by enzyme linked immunosorbent assay and passive cutaneous anaphylaxis assay. In OVA and HEWP groups,significantly high titres of specific IgE (1∶32) were provoked compared with that in the control group at the 28th and 42nd day. However, at the 14th, 28th, and 42nd day in PAP group and at the 14th day in OVA and HEWP groups, the difference of specific IgE between test groups and the control group was not significant. BN rats could be made allergic to OVA and HEWP(major allergenic protein), and could not be made allergic to PAP( non allergenic protein). It is concluded that BN rats may be a fairly good animal model suitable for the investigation of food allergy.