食品中转基因大豆成分的定性和定量检测

Qualitative and quantitative detections of transgenic component in genetically modified soybean using real-time PCR

为建立食品中转基因成分的定性定量分析 ,运用传统的定性PCR方法检测大豆加工产品中转基因成分的存在 ,运用Taqman探针技术 ,对存在的转基因成分进行准确定量。对于定量过程中由于扩增效率的不同造成的误差 ,通过大豆的内源基因Lectin进行校正 ,根据ΔCt值对应于校正曲线计算出转基因大豆的含量。本方法灵敏度达到 0 1% ,误差范围小于 30 0 %。可用于转基因大豆的监测管理。

Many countries have constituted higher requirement to the detection of GM products since a series of labeling regulations were put into practice. This requires accurate quantification of the transgenic material in the GM products. A conventional qualitative polymerase chain reaction(PCR)assay was used to detect the presence of GM soy and a real time PCR was used to quantify the content of GM soy.The errors due to difference in amplification efficiencies were amended by detecting the endogenous control.The percentage of genetically modified soybean was calculated by ΔCt value through a standard curve.The sensitivity of the method could reach 0 1% and the range of error was less than 30 0%.