三唑磷对麦穗鱼脑组织中乙酰胆碱酯酶的诱导

Induction of Triazophos to Brain Acetylcholinesterase from Topmouth Gudgeon,Pseudorasbora parva

为了检测有机磷杀虫剂对鱼脑组织中乙酰胆碱酯酶 (AChE, EC3.1.1.7)的诱导,采用间接非竞争酶联免疫吸附测定法(ELISA)检测了三唑磷胁迫下麦穗鱼Pseudorasboraparva脑组织中乙酰胆碱酯酶含量的变化。当暴露浓度仅为0.1μg/L时,三唑磷即对AChE产生诱导。由于存在诱导效应,三唑磷对酶活性的抑制在一定程度上被酶蛋白含量的提高所掩盖。研究结果表明,以酶蛋白替代总蛋白来衡量粗酶液中AChE的活性,可以明显提高对有机磷胁迫作用的检测灵敏度。

For the purpose of detecting of induction of acetylcholinesterase (AChE, EC 3.1.1.7) by (organophoshorous) insecticides, amount of brain AChE of a freshwater fish, i.e., (topmouth) (gudgeon ), Pseudorasbora parva, which underwent exposure of triazophos, was detected using an (indirect) non-competitive ELISA. The in vivo AChE was (induced) at such a low concentration of (0.1 μg /L.) Due to the induction inhibition on activity of AChE by (triazophos) was masked, in some extent, by the increase of the enzyme content. Result of the present (research) indicated that a sensitive detecting of exposure could be achieved if (specific) activity of AChE was evaluated by the enzyme content, rather than by the total protein (content) of the crude (extract.)