摘要
为了检测有机磷杀虫剂对鱼脑组织中乙酰胆碱酯酶 (AChE, EC3.1.1.7)的诱导,采用间接非竞争酶联免疫吸附测定法(ELISA)检测了三唑磷胁迫下麦穗鱼Pseudorasboraparva脑组织中乙酰胆碱酯酶含量的变化。当暴露浓度仅为0.1μg/L时,三唑磷即对AChE产生诱导。由于存在诱导效应,三唑磷对酶活性的抑制在一定程度上被酶蛋白含量的提高所掩盖。研究结果表明,以酶蛋白替代总蛋白来衡量粗酶液中AChE的活性,可以明显提高对有机磷胁迫作用的检测灵敏度。
For the purpose of detecting of induction of acetylcholinesterase (AChE, EC 3.1.1.7) by (organophoshorous) insecticides, amount of brain AChE of a freshwater fish, i.e., (topmouth) (gudgeon ), Pseudorasbora parva, which underwent exposure of triazophos, was detected using an (indirect) non-competitive ELISA. The in vivo AChE was (induced) at such a low concentration of (0.1 μg /L.) Due to the induction inhibition on activity of AChE by (triazophos) was masked, in some extent, by the increase of the enzyme content. Result of the present (research) indicated that a sensitive detecting of exposure could be achieved if (specific) activity of AChE was evaluated by the enzyme content, rather than by the total protein (content) of the crude (extract.)
出处
《农药学学报》
CAS
CSCD
2005年第1期59-62,共4页
Chinese Journal of Pesticide Science
基金
浙江省自然科学基金(No. 392043).