血清丙氨酸氨基转移酶、总蛋白定量分析方法的评价

Estimation of Quantitative Analysis Method for Serum ALT and Total Protein

目的 对现行使用的BeckmanDeltaCX7全自动生化仪的性能和试剂进行定量试验方法的评估。方法 采用美国标准化委员会的标准化文件EP10 T2 ,选择具有代表性的丙氨酸氨基转移酶 (ALT)、血清总蛋白 (TP) ,采用酶法测定ALT ,双缩脲法测定TP。结果 测定高、中、低不同浓度质控液 ,计算批内偏差、允许误差以及总不精密度和允许不精密度 ,符合仪器检测准确度和精密度要求。结论 对该仪器整体性能进行的论证结果表明 ,BeckmanDeltaCX7全自动生化分析仪目前的各项性能良好 ,用该仪器分析ALT和TP ,其结果是可靠的。

Objective To estimate the function of Beckma n DeltaCX7 automatic biochemistry instrument and the quantitative method for the reagent which is used at present.Method U.S Standardization Com mission's standard file EP10-T2 was used to select representative ALT and TP.AL T was determined with the enzyme reaction,TP was determined with biuret reaction .Results The high,middle,low different consistency quality cont rol liquid were determined,and the error,admissible deviation and total imprecis ion,as well as admissible imprecision were calculated,which were conformed to th e demand of the instrument's determination.Conclusions The func tion of Beckman DeltaCX7 automatic biochemistry instrument is fine.The determina tion results of ALT and TP with it are reliable.