番茄果实蔗糖磷酸合成酶基因的克隆与序列分析

Cloning and sequencing of Tomato fruit sucrose phosphate synthase gene

根据GenBank中已登记的番茄、马铃薯等蔗糖磷酸合成酶基因的保守序列设计特异引物,采用RT-PCR方法,从番茄自交系20-19果实总RNA中克隆到目的基因的全长 cDNA。序列分析表明,它与Gen Bank中登记号为AB051216的番茄蔗糖磷酸合成酶基因高度同源,核苷酸序列的同源性为98%。该基因已在GenBank中注册,登记号为AY726439。

PCR primers were designed based on the consensus domain of some sucrose phosphate synthase genes registered in GenBank. A aimed complete cDNA was obtained by using RT-PCR from tomato fruit . The result of testing sequence showed that it shared 98% homology in nucleotide sequence with tomato sucrose phosphate synthase gene ,whose access number is AB051216. This gene had been registered in GenBank.The access number is AY726439.