转基因八棱海棠与苹果品种亲和性的微嫁接早期鉴定

Early identification of graft compatibilities between Malus micromalus and apple cultivars by micrografting in vitro

用微嫁接法鉴定嫁接亲和性的结果表明,不同培养基和培养方法对嫁接成活率和生根率有较大影响,生根 愈伤同步的方法可以同时获得较高的嫁接成活率和生根率。转番茄铁载体蛋白基因八棱海棠(Malus micromalus)与 苹果(Malus pumila)品种富士,嘎拉以及新红星嫁接成活率分别为93.33％,92.86％,73.34％。嫁接后15 d左右,接穗 新叶开始生长;嫁接后2个月,嫁接苗移栽成活率分别为73.07％,73.91％和55％;移栽后3个月,3种嫁接苗的茎杆 粗度均匀,无大小脚现象。初步结果显示,转基因八棱海棠与富士、嘎拉均具良好的嫁接亲和性,而与新红星的嫁接 亲和性稍差。

The graft compatibilities between transgenic Malus micromalus and apple cultivar: Fuji, Gala and Starkrimson, were identified by in vitro micrograft techniques. The results showed that the survival percentages were 93.33%, 92.86% and 73.34% respectively. About 15 days after micrografted, the new leaves of scion cultivar expanded. Two months later, they were transplanted, their survival rate were 73.07%, 73.91% and 55%. It is believed that the graft compatibility of transgenic M. micromalus with Fuji and Gala apple cultivars was good, but poor with Starkrimson. Results also showed that medium and grafting method obviously affected the survival rate and rooting ability.