尼莫地平孕期干预对生长受限仔鼠神经系统损伤的保护作用

Protective Effect of Nimodipine Treatment during Gestation on Neural Injury for Rats Complicated with Fetal Growth Restriction

【目的】探讨钙离子拮抗剂孕期干预能否减轻生长受限仔鼠神经系统损伤。【方法】将40只SD大鼠按妊娠先后顺序随机分为3组:①A组:不缩窄子宫动脉,不予尼莫地平治疗;②B组,缩窄子宫动脉,不予尼莫地平治疗;③C组:缩窄子宫动脉,术后予尼莫地平治疗。通过TUNEL法检测各组新生仔鼠皮层和海马CAl 区神经元凋亡;Morris水迷宫法检测出生21 d后各组仔鼠学习记忆功能。【结果】①3组仔鼠皮层神经细胞凋亡率差异有统计学意义(F=55.63,P<0.01)。其中C组仔鼠平均为(2.98±0.54)个/HP,低于FGR组(5.05±1.17)个/ HP(P<0.01),但高于对照组(2.30±0.69)个/HP(P<0.05)。②3组仔鼠海马CA1区神经元凋亡率差异有统计学意义(H=34.88,P<0.01)。其中C组中位数为0.833(0.332)个/HP,低于B组1.5(0.75)个/HP(P<0.01),高于A 组0.5(0.333)个/HP(P<0.01)。③Morris水迷宫学习记忆功能检测,3组仔鼠5 d的训练差异有统计学意义,F (组间)=44.052,P<0.01;F(时间)=7 153.846,P<0.01)。C组仔鼠找到平台的时间较B组短(P<0.05),但较A 组长(P<0.05)【结论】钙离子拮抗剂尼莫地平孕期治疗能对生长受限仔鼠神经系统损伤有保护作用。

[Objective]To study whether calcium channel antagonist treatment during gestation can alleviate the neural system damage. [Method] Forty SD rats were randomly divided into 3 groups:(1)group A: without uterine artery ligation,without Nimodipine treatment;(2)group B:partial occlusion of bilateral uterine artery,without nimodipine treament.(3)group C:partial occlusion of bilateral uterine artery,with nimodipine treament after operation. Neuronal apoptosis in cortex and hippocampus of newborn pups in each group were detected by TUNEL. The pups' learning-memory ability was tested by Morris water maze task on the 21st day after birth. [Result](1)The ratio of neuronal apoptosis in cortex of each group was statistically different (F=55.63,P<0.01). It was (2.98±0.54)/ HP in group C, lower than that of group B (5.05±1.17)/HP,(P<0.01),but higher than that of group A (2.3±0.69) /HP,(P<0.05).(2)The ratio of neuronal apoptosis in hippocampus in each group was statistically different (H= 34.88,P<0.01). The media in group C was 0.833 (0.332)/HP,lower than that of group B, 1.5 (0.75)/HP (P< 0.01), but higher than that of group A, 0.5(0.333)/HP(P< 0.01).(2)The results of Morris water maze showed that it was statistically different among three groups(F of groups=44.052, P<0.01;F of time=7153.846, P<0.01). In group C, the average escape latency was shorter than group B, but longer than group A. [Conclusion] Calcium channel antagonist nimodipine treatment during gestation has a protective effect on neural injury induced by FGR.