动脉粥样硬化小型猪三磷酸腺苷结合盒转运体A1表达的变化

Change of ATP Binding Cassette Transporter A1 Expression in Atherosclerotic Chinese Minipigs

用贵州小香猪建立动脉粥样硬化动物模型,探讨动脉粥样硬化小型猪三磷酸腺苷结合盒转运体A1(ABCA1)表达的变化. 采用血管内膜损伤法加高脂高胆固醇饲料喂养贵州小香猪,建立动脉粥样硬化动物模型. 血浆总胆固醇、甘油三酯和高密度脂蛋白胆固醇的浓度均用氧化酶法测定,采用逆转录聚合酶链反应检测ABCA1mRNA水平,蛋白质印迹和免疫组织化学检测ABCA1蛋白质的表达. 喂养12个月后,实验组与正常对照组比较,空腹血浆总胆固醇、甘油三酯和高密度脂蛋白胆固醇水平升高;实验组小型猪主动脉、髂动脉、颈总动脉和冠状动脉可见动脉粥样硬化斑块和脂质条纹;实验组小型猪肝组织、主动脉、小肠组织ABCA1表达上调. 结果提示,采用血管内膜损伤法加高脂高胆固醇饲料喂养小型猪可建立动脉粥样硬化动物模型. 动脉粥样硬化小型猪肝组织、主动脉和小肠组织ABCA1表达上调.

In order to establish an animal model of atherosclerosis in minipigs and investigate the change of ATP binding cassette transporter A1(ABCA1) expression in atherosclerotic minipigs, Chinese minipigs were fed a normal control diet (CD) or a high fat/high cholesterol diet (HFHC) and carotid overstretch ballon injury for 12 months. Plasma total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and triglycerides (TG) were determined by commercially enzymatic methods. ABCA1 mRNA and protein level were determined by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry, respectively. At the end of 12 months, plasma total cholesterol, HDL cholesterol and triglyceride in atherosclerotic minipigs were increased. It is obvious that atherosclerotic plaques and lipid stripes in aonta, iliaca anteria and coronary artery of atherosclerotic minipigs ATP binding cassette transporter A1 expression in liver, aorta and small intestine of atherosclerotic minipigs was upregulated. HFHC may induce atherosclerosis and upregulation of ATP binding cassette transporter A1 expression in atherosclerotic minipigs.