食管癌表达上调基因NADH氧化还原酶MLRQ亚基的研究及临床意义

Expression of MLRQ subunit gene of NADH oxidoreductase and its clinical significance in malignant tumors of digestive system

目的研究在食管癌中差异表达明显的NADH氧化还原酶MLRQ亚基基因在食管癌中的表达,并探讨它在食管癌发生发展过程中的可能机制。方法利用微陈列技术方法获得一个在食管癌中的表达显著上调的NADH氧化还原酶MLRQ亚基基因,用RT PCR及Northern印迹从mRNA水平检测其在38对食管癌、7对贲门癌、14对胃癌、11对结肠癌和7对肝癌等消化系统肿瘤中的表达情况。结果(1)MLRQ基因在食管癌、贲门癌、胃癌、结肠癌和肝癌中显著上调者,分别为81.6%(31/38)、57.1%(4/7)、85.7%(12/14)、65.6%(7/11)、57.1%(4/7)。用X2检验,各组之间差异无统计学意义(P>0.05)。(2)在38对食管癌配对组织中,MLRQ基因上调与临床分期、肿瘤浸润程度、淋巴结转移及分化程度无相关性(均P>0.05)。结论MLRQ亚基基因在消化道肿瘤中表达上调。

Objective To study the differential expression and possible role of MLRQ subunit gene of nicotinamide adenine dinucleotide reduced (NADH) oxidoreductase in malignant tumors of digestive system. Methods Specimens of cancerous tissues and matched adjacent normal tissues resected during operation or biopsy: 38 pairs of specimens of esophageal carcinoma, 7 pairs of specimens of cardiac carcinoma, 14 pairs of specimens of gastric carcinoma, 11 pairs of specimens of colon carcinoma, and 7 pairs of specimens of liver carcinoma underwent PCR test and Northern hybridization to detect the differential expression of MLRQ subunit gene of NADH oxidoreductase. Results (1) Overexpression of MLRQ subunit gene was found in 31 of the 38 pairs of specimens of esophageal carcinoma (81.6%), 4 of the 7 pairs of specimens of cardiac carcinoma (57.1%), 12 of the 14 pairs of specimens of gastric carcinoma (85.7%), 4 of the 7 pairs of specimens of colon carcinoma (65.6%), and 7 of the 11 pairs of specimens of liver cancer (57.1%). No significant difference among different cancers was observed by X2 test (all P>0.05). (2) The up-regulation of MLRQ subunit was not correlated with clinic stage, infiltration degree, lymphatic metastasis, and differentiation of tumor (all P>0.05). Conclusion MLRQ subunit gene is up-regulated in the malignant tumors of digestive system.