牛血清免疫球蛋白的盐析法提取与纯化

Studies on extraction and purification methods of saltingout IgG from bovine serum

选取饱和硫酸铵、辛酸-硫酸铵、饱和硫酸钠三种盐析方法,对牛血清中的免疫球蛋白进行分离提取,得到免疫球蛋白的粗提物。通过比较三种方法的提取得率和产品回收率确定采用饱和硫酸铵沉淀法提取效果最佳。其提取得率为9.06g/L,产品回收率为91%,是最适合对血清中免疫球蛋白进行粗提的盐析方法。

In this research subject, the crude extraction of the Ig in bovine serum is used by three neuter salts, which include ammonium sulphate, caprylic acid ammonium sulphate, saturated sodium sulfate. And then to compare the extracted formation and the of recover experiments among these three salts out methods, the result shows that saturated ammonium sulphate precipitate is the best crude extraction method whose extracted output is 9.06mg/ml and the recovery of Ig is 91%.