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叶片衰老抑制基因P_(SAG12-ipt)转入籼稻不育系中A的研究 被引量：2

Studies of the Leaf-specific Senescence-inhibitor Gene (P_(SAG12-ipt)) Introduced into Zhong A (Cytoplasmic Male Sterile Indica)
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摘要 以质粒pSG516(ipt)为目的基因,pHQ9(hpt和gus)为选择 标记基因进行共转化,以PSD-1000-氦气基因枪介导,将ipt、hpt和gus基因转化到籼型不育系中A的核DNA中,得到了98个潮霉素抗性再生植株系。经过GUS基因组织化学染色检测、PCR检测以及Southernblot杂交分析,证实了叶片衰老抑制基因PSAG12-ipt已经整合到水稻基因组中。 pSG516(ipt) and pHQ9(hpt and gus)were co-transferred into Zhong A (cytoplasmic male sterile indica) genomic DNA by the particle bombardment of Biolistic PSD-1000/He Particle Delivery System (BIO-RAD) with the plasmid DNA mixed to Gold Particle. Ninety-eight independent lines were obtained by selecting for hygromycin resistance. Results of GUS, PCR and Southern blot demonstrated that the leaf-specific senescence-inhibitor gene (P_(SAG12-ipt)) has been integrated into rice genome.
作者 王亚琴 梁承邺
机构地区 华南理工大学生物科学与工程学院 中国科学院华南植物研究所
出处 《中山大学学报（自然科学版）》 CAS CSCD 北大核心 2005年第2期78-81,共4页 Acta Scientiarum Naturalium Universitatis Sunyatseni
基金 广东省重大科技专项基金资助项目(2003B21207)
关键词 叶片衰老 GUS基因 籼稻 籼型不育系 潮霉素 株系 杂交分析 抑制基因 组织化学染色 介导 leaf-specific senescence-inhibitor gene (P_(SAG12-ipt)) particle bombardment Zhong A (cytoplasmic male sterile indica)
分类号 S511 [农业科学—作物学] S513 [农业科学—作物学]
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参考文献12

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同被引文献20

  • 1王亚琴,梁承邺.转P_(SAG12-ipt)基因水稻延衰性能的初步研究[J].广西植物,2004,24(6):540-543. 被引量：9
  • 2李柏林,梅慧生.燕麦叶片衰老与活性氧代谢的关系[J].植物生理学报（0257-4829),1989,15(1):6-12. 被引量：345
  • 3Chen LFO,Hwang JY,Charng YY,etal. 2001. Transformation of broccoli (Brassica olearacea var. italica) with isopentenyl transferase gene via Agrobacterium tume facien for postharvest yellowing retardation[J]. Mol Breeding,7:243-257.
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  • 10McCabe MS,Garratt L C,Schepers F,et al. 2001. Effects of PSAG12-IPT gene expression on development and senescence in transgenic lettuce[J]. Plant Physiol, 127: 505-516.

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中山大学学报（自然科学版）
2005年 第2期

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