三仙汤对去卵巢大鼠骨密度和钙磷代谢的影响

Influences of Sanxian decoction on bone mineral density and metabolism of calucium and phosphor in ovariectomized rats

目的 通过测定去卵巢大鼠骨质疏松症模型骨密度 (BMD)、血清中钙、磷、碱性磷酸酶 (ALP)及尿钙、尿磷 ,了解三仙汤是否能改善去卵巢大鼠骨质疏松症 ,并进一步探讨其起效的具体机制。方法3月龄雌性SD大鼠 5 9只 ,随机分为 6组 :假手术组 (Sham)、模型组 (OVX)、尼尔雌醇组 (Nil)、三仙汤小、中、大剂量组。Sham组、OVX组用生理盐水灌胃。Nil组以 0 15mg/ 10 0g大鼠体重给药 ,三仙汤小、中、大组给药量分别为 5 98g/kg、11 96g/kg及 2 3 92g/kg。除Nil组每周灌胃一次外 ,其他组均每周给药 6次。持续 3月后处死大鼠 ,测定其股骨BMD及血清中钙、磷、ALP及尿钙、磷。结果 OVX组大鼠BMD较Sham组显著降低 (P <0 . 0 1) ,三仙汤各组及Nil组与OVX组比较均明显上升 (P <0 . 0 1) ;OVX组血钙、血磷浓度与Sham组比较有显著下降 (P <0 . 0 1或P <0 . 0 5 ) ,三仙汤各剂量组、Nil组与OVX比较则均显著上升 (P <0 . 0 1或P <0 . 0 5 ) ;OVX组血清ALP浓度较Sham升高 (P >0 . 0 5 ) ,三仙汤各组ALP含量与OVX组比较明显上升 (P <0 .0 5 ) ,而Nil组反而明显降低 (P <0. 0 5 )。OVX的尿钙、尿磷均较Sham组明显升高 (P <0 . 0 5 ) ,三仙汤各组尿钙与OVX组比较有下降趋势 (P >0 . 0 5 ) ,尿磷则明显下降 (P <0 . 0 1或P

Objective To study the effects of Sanxian decoction on the bone mineral density (BMD), serum calcium, and phosphor, urine calcium, and phosphor, and serum alkaline phosphoric acid (ALP) in ovariectomized rats. Methods Fifty-nine 3-month-old female SD rats were randotmized into six groups: sham-OVX group; ovariectomized group (OVX); nilestriol group; low dosage Sanxian decoction; moderate dosage Sanxian decoction; and high dosage Sanxian decoction. The experiment lasted for three months. At the end of experiment, BMD, serum calcium, and phosphor, urine caIcium, and phosphor, and ALP in ovariectomized rats were determined.Results The BMD in OVX group was 0.372 g/cm^2, significantly lower than that of 0.416 g/cm^2 in Sham-OVX group (P<0.01), and the BMD in all the three Sanxian groups and nilestriol group were significantly higher than thatin OVX-group(P<0.01), The serum calcium, and phosphor in OVX-group were significantly lower than that in sham-OVX group (P<0.05 or P<0.01), while those in the three Sanxian decoction groups and nilestrioli group were significantly higher than that in OVX-group (P<0.05 or or P<0.01), The ALP content in OVX-group showed an increasing trend but did not so in sham-OVX group, and the ALP contents in the three Sanxian decoction groups were significantly higher than that of OVX-group, while that in the nilestriol group was significantly decreased (P<0.05), the urine calcium, and phosphor in OVX-group were significantly higher than those in Sham-OVX group (P<0.05), and the urine calcium in Sanxian decoction groups showed an decreasing trend but did not so in OVX-group, while the urine phosphor in Sanxian groups were significantly lower than that in OVX-group, even lower than that in nilestrioli group (P<0.01).Conclusions Sanxiari decoction raises the BMD in ovariectomized rats, maybe by increasing the calcium, phosphor, and ALP, and reducing the urine calcium, and phosphor in ovariectomized rats.