广西东兰县壮族长寿老人HLA-DQA1和HLA-DQB1基因的多态性

Polymorphism of HLA-DQA1 and HLA-DQB1 loci of Chuang nationality longevous population in Donglan County of Guangxi

目的了解长寿老人的人类白细胞抗原-DQA1、DQB1(HLA-DQA1、HLA-DQB1)基因多态性的特点,探讨HLA与长寿的相关性.方法应用聚合酶链式反应-序列特异引物(PCR-SSP)对东兰县150名90岁以上壮族长寿老人(90～105岁)和143名当地健康、无血缘关系、随机抽样的壮族成年人(20～76岁)进行HLA-DQA1和HLA-DQB1两座位的基因分型并进行相应等位基因频率的比较.结果①长寿组等位基因HLA-DQA1*0102的频率明显高于对照组(分别为41.33%和28.32%,P<0.05);②长寿组HLA-DQA1*0101及HLA-DQB1*0301的频率分别为3.67%和9.00%,明显低于对照组的13.64%和21.68%(P均<0.01).③其余各相应等位基因频率之间的比较无显著差异(P值均＞0.05).相关分析显示,HLA-DQA1*0102与长寿呈正相关,而DQA1*0101及DQB1*0301呈负相关(OR分别为1.308 3、0.241 0及0.329 6;P分别＜0.05、0.01及0.01).结论在东兰县壮族人群中,带有HLA-DQA1*0102等位基因的个体可能有利于长寿,而HLA-DQA1*0101和DQB1*0301则可能是长寿的不利因子.

Objective To investigate correlationship between HLA and longevity by analyzing the polymorphism of HLA-DQA1 and-DQB1 loci. Methods HLA-DQA1 and-DQB1 were genotyped with Polymerase Chain Reaction-sequence specific primer (PCR-SSP) method for 150 longevous Chuang nationality people (90~105 years) and 143 unrelated healthy Chuang nationality people selected randomly (20~76 years old) in the same area and compared on corresponding allele frequency. Results ①The frequency of HLA-DQA1*0102 allele in the longevous group was significantly higher than that in control group (41.33% and 28.32%, respectively, P<0.05). ②The frequency of HLA-DQA1*0101 and HLA-DQB1* 0301 (3.67% and 9.00%, respectively) were much lower than those in control group (13.64% and 21.68%, both P<0.01). ③There was no significant difference in other alleles between the two groups (P>0.05 for all). Correlation analysis showed that HLA-DQA1*0102 had positive (OR=1.308 3, P<0.05) while HLA-DQA1*0101 and DQB1*0301 had negative (OR=0.241 0 and 0.329 6, respectively, both P<0.01) relationship to longevity. Conclusions HLA-DDQA1*0102 allele might be in favor of longevity in Chuang nationality people in Donglan County, but HLA-DQA1*0101 and-DQB1*0301 might be disadvantageous factor.