蝙蝠葛酚性碱对家兔心脑缺血-再灌注损伤保护作用的研究

Protective effect of phenolic alkaloids from Menispermum dauricum on myocardial and cerebral ischemia/ reperfusion injury in rabbits

目的 探讨蝙蝠葛酚性碱 (PAMd)抗心、脑同时缺血再灌注损伤的机制。方法 采用结扎家兔左冠状动脉前降支及双侧颈总动脉 30 m in后再灌注造成心、脑缺血再灌注模型 ,观察 PAMd对缺血前后及再灌注后不同时间血清、左心室、海马、皮质及小脑中丙二醛 (MDA)含量和超氧化物歧化酶 (SOD)活性的影响。结果 家兔心、脑缺血再灌注 10 min后血清 MDA含量显著升高 ,SOD活性显著降低 (P均 <0 .0 5 )。PAMd3.5 mg/ kg显著降低心、脑缺血再灌注后血清及组织 MDA含量 ,升高 SOD活性 (P均 <0 .0 5 )。结论PAMd通过减轻脂质过氧化所造成的损伤 ,提高 SOD活性 ,对心、脑缺血再灌注损伤具有一定保护作用。

Objective To explore the mechanism underlying the effects of phenolic alkaloids abstracted from Menispermum dauricum (PAMd) on concurrent myocardial and cerebral ischemia/reperfusion injury in rabbits . Methods Both left anterior descending coronary artery and bilateral carotid arteries were ligated to induce myocardial and cerebral ischemia/reperfusion injury at the same time in rabbits. At 30 minutes after ischemia, the occlusion was released to allow return of circulation, and 2 ml of blood was collected from the femoral vein 10 minutes before ischemia, 1, 10, 30 minutes after ischemia, and 1,10, 30, 60, 120, 180, 240 minutes after reperfusion. All rabbits were sacrificed at the end of reperfusion, and the left ventricle, hippocampus , cortex, and cerebellum were removed for examination. Malondialdehyde (MDA) content and superoxide dismutase(SOD) activity were determined. Results At 10 minutes after reperfusion, MDA content in serum was significantly higher and SOD activity was significantly lower in ischemia/reperfusion group than those of control group (both P <0 05). In rabbits with administration of PAMd, MDA content was lower and SOD activity was higher in serum compared with ischemia/reperfusion group( P <0 05). Similar results as in the blood in regard to MDA content and SOD activity were obtained in tissues. Conclusion PAMd could attenuate the injury induced by lipid peroxidation and enhance the activity of SOD, thus it might play a protective role in concurrent myocardialcerebral ischemia/reperfusion injury.