扶正养营汤对免疫介导再生障碍性贫血小鼠的治疗作用

Therapeutic effect of fuzheng yangying oral solution on immune-induced aplastic anemia mice

目的:观察扶正养营汤对免疫介导再生障碍性贫血(再障)小鼠骨髓增殖及外周血IL 2水平的影响 方法:采用完全随机数字表法将制作的免疫介导再障小鼠(BALB/c)模型40只分为4组,另用10只同批正常小鼠 设为正常对照组(E组)。采用盲法分别胃饲生理盐水(A组及E组)、扶正养营汤(B组)、环孢霉素A(C组)、扶 正养营汤+环孢霉素A(D组)共10d。检测各组小鼠外周血象、骨髓有核细胞计数、测定各组骨髓造血组织容量 及外周血IL 2水平。结果:A组小鼠外周血象、骨髓有核细胞数、造血组织容量显著低于E组(P<0.05);B,C, 组较A组增加(P<0.05),尤以D组最为明显(P<0.05);而B组与C组比较差异无统计学意义(P>0.05)。A组 小鼠外周血IL 2水平明显高于E组(P<0.05);B,C,D组低于A组(P<0.05);D组低于B组及C组(P<0.05) B组与C组比较差异无统计学意义(P>0.05)。结论:扶正养营汤具有促进免疫介导再障小鼠骨髓增殖,降低其外 周血IL 2水平的作用。

Objective To observe the effect of fuzheng yangying oral solution on bone marrow proliferation and serum interleukin 2 in immune-induced aplastic anemia mice. Methods The immune-induced aplastic anemia mouse models were built up, and then were divided into 4 groups by randomized allocation. The models were fed with physical salts (Group A, n=10 ), fuzheng yangying oral solution (Group B, n=10), cyclosporin A (Group C, n=10), and fuzheng yangying oral solution plus cyclosporin A (Group D, n=10), respectively. Another 10 healthy mice served as controls (Group E). The changes in blood cells, bone marrow karyocyte count, marrow hematopoietic tissue capacity, and serum interleukin 2 level were observed. Results The blood cells, bone marrow karyocyte count, and marrow hematopoietic tissue capacity in Group A were significantly lower than those of Group E (P< 0.05) ; those in the 3 treated groups (Group B, C, and D) were significantly higher than those of Group A (P<0.05); and Group D was the highest (P<0.05). There were no significant difference between Group B and C (P>0.05). The level of interleukin 2 in Group A was obviously higher than that of Group E (P<0.05); The levels of interleukin 2 in the 3 treated group (Group B, C, and D) were significantly lower than those of Group A (P<0.05); and Group D was the lowest (P<0.05). There was no significant difference in the level of interleukin 2 between Group B and C (P<0.05). Conclusion Fuzeng yangying oral solution can impove the bone marrow proliferation and reduce the level of serum interleukin 2 in immune-induced aplastic anemia mice.