慢性前列腺炎患者前列腺液中IL-2、IL-8及IL-10水平分析

Analysis of Cytokines (IL-2, IL-8, IL-10) in the Expressed Prostatic Secretions of Chronic Prostatitis

目的: 检测慢性前列腺炎(CP)患者前列腺液中细胞因子IL 2、IL 8及IL 10的水平,探讨这些细胞因子对CP发病机制及诊断方面的价值。 方法: 采用双抗体夹心ELISA法测定 31例CP患者前列腺液中IL 2、IL 8及IL 10的水平,并以 10例健康男性为对照。对每例患者进行两杯法尿液细菌培养、前列腺常规检查和美国国立卫生院前列腺炎症状指数评分 (NIH CPSI)。按NIH分类法将 31例CP患者分为 3型:Ⅱ型 5例,ⅢA型 13例,ⅢB型 13例。 结果: CP组与对照组比较,前列腺液IL 8含量显著升高(P<0. 05),IL 2和IL 10含量显著降低 (P均<0. 05)。Ⅱ型CP组前列腺液IL 2、IL 8和IL 10水平与ⅢA型CP组比较,差异均无显著性。ⅢB型CP组与Ⅱ型和ⅢA型CP组比较,前列腺液IL 8水平显著降低(P均<0. 05)。CP患者前列腺液IL 8水平与IL 10水平呈负相关(r=-0. 503,P<0. 05),与前列腺液白细胞计数呈正相关(r=0. 663,P<0. 05)。前列腺液IL 2、IL 8和IL 10水平与NIH CPSI评分无相关性(P>0. 05)。 结论: 前列腺液中IL 2、IL 8及IL 10在CP的发病过程中起重要作用,是诊断CP有价值的指标。

Objective: To investigate the levels of immunoregulatory cytokine IL-2, pro-inflammatory cytokine IL-8 and anti-inflammatory cytokine IL-10 in the expressed prostatic secretions (EPS) of chronic prostatitis (CP) patients and to evaluate the significance of the cytokines to the pathogenesis and diagnosis of CP. Methods: IL-2, IL-8 and IL-10 levels were measured in the EPS of 31 CP patients and 10 normal controls by enzyme-linked immune sandwich assay(ELISA). Urine was culturedand EPS studied according to the 2-glass test. NIH-CPSI(NIH-chornic prostatitis symptom index) was performed in every patient. The cases of CP were divided into 3 types: Ⅱ (n=5), ⅢA(n=13) and ⅢB(n=13) according to NIH. Results: The IL-8 levels in CP patients were significantly higher(P< 0.05) while the IL-2 and IL-10 levels significantly lower than those in the controls(both P< 0.05). There was no statistically significant difference between the cytokine levels in Ⅱ CP and in ⅢA CP(P> 0.05). The IL-8 levels in ⅢB CP were significantly lower than those in both Ⅱ CP and ⅢA CP(both P< 0.05). The IL-8 levels were correlated directly with WBC count (r= 0.663,P< 0.05), and inversely with IL-10 levels (r=- 0.503,P< 0.05), but there was no correlation between NIH-CPSI scores and cytokines levels(P> 0.05). Conclusion: Cytokines may play an important role in pathogenesis of prostatitis. The results indicate that the expression of cytokines(IL-2, IL-8, IL-10) in EPS can serve as a valuable marker for the diagnosis of CP.