红松体细胞胚胎培养技术体系的建立

Somatic Embryogenisis (SE) in Korean Pine (Pinus.Koraiensis Sieb. et Zucc)

以5个红松家系未成熟合子胚为材料,首次建立了红松体细胞胚胎(somaticembryo,SE)培养体系,为红松人工种子培育苗及基因转化奠定了基础实验表明:在GLH培养基上黑暗培养约6周后,5个红松家系未成熟胚的SE诱导率分别为46.31%、28.2%、26.57%、17.81%、9.652% 红松遗传类型、合子胚发育程度对SE诱导率影响均达到极显著的水平;遗传类型和合子胚发育程度之间的交互作用达到极显著水平,但合子胚发育程度与培养基类型间。

For the first time, somatic embryogenic system was established, utilizing five families of Pinus.Koraiensis Sieb. et Zucc as materials, which settled the basis for its artificial seeds production and gene transformation. Our results demonstrated that somatic embryos (SE) could be induced from P. Koraiensis immatured embryos cultured on GLH media in dark for about 6 weeks. The SE initiation frequencies were 46.31%、28.2%、26.57%、17.81%、9.652%, respectively. The genotype and development stage of P. Koraiensis zygotic embryos were the most important factors influencing the SE initiation frequency, and correlation coefficients (CC) between the two factors was great distinguished. While CC between genotype and culture media, and CC between development stage of zygotic embryos and culture media were negligible.