糖尿病鼠血清及睾丸中一氧化氮含量和一氧化氮合酶活性的变化

Changes in NO content and NOS activity in serum and testis in diabetic rat

目的 研究1型糖尿病大鼠血清和睾丸中一氧化氮(NO)含量和一氧化氮合酶(NOS)活性的动态变化。方法 63只 Wistar雄性大鼠随机分为对照组(C)和糖尿病组(D),采用四氧嘧啶腹腔注射复制糖尿病动物模型。糖尿病组分别于观察1、2、3、4、5、 6、7和8周后断头处死动物,取血收集血清,取睾丸制备组织匀浆,分别测定NO含量和NOS活性。结果 与对照组比较,糖尿病组 血清NO含量升高,NOS活性先升后降;睾丸组织在诱模后NO含量下降,而NOS活性变化不明显,随后两者均呈升高趋势。结论 糖尿病大鼠血清和睾丸NO含量和NOS和活性均有改变。

Objective To observe the changes in NO content and NOS activity of serum and the testis in Wistar rats with type I diabetes mellitus. Methods 63 rats were randomly divided into control group(C) and diabetes group(D). Diabetes was induced by intraperitoneal injection of Alloxan. Rats with diabetes were observed for 1 week(D 1), 2 weeks(D 2), 3 weeks(D 3), 4 weeks(D 4), 5 weeks(D 5), 6 weeks(D 6), 7 weeks(D 7) and 8 weeks(D 8), respectively, and they were sacrificed at the end of each observation period. Serum was obtained and testis homogenates were prepared, and the levels of NO content and NOS activity of them were determined respectively. Results Compared diabetes group with control group, NO contents and NOS activity of serum from diabetic rats were higher, then NOS activity began to decline to approach the control level. In the testis homogenates, NO content dropped slightly after injection of Alloxan, while NOS activity showed no significant change. Thereafter, both NO content and NOS activity of testis homogenates were increased. Conclusion There are changes in NO content and NOS activity of serum and testis of rats after diabetes are induced with Alloxan.