大鼠原位肝移植动物模型制作要点

Method of Orthotopic Liver Transplantation in Rats

目的 建立稳定的大鼠原位肝移植模型。方法 参照 Kamada等法建立大鼠原位肝移植模型,经门静脉灌注肝脏,改进肝上下腔静脉吻合法为单线连续缝合法。结果 210只大鼠原位肝移植24 h存活率为91.0%(191/210),平均无肝期17 min,1周生存率为85.2%(179/210)。结论 改进大鼠原位肝移植肝上下腔静脉吻合法,可缩短受体无肝期,减少手术并发症发生率,并能提高原位肝移植大鼠的生存率。

Objective To establish the stable model of orthtopic liver transplantation in rats.Methods In the light of Kamada’s method, the donor’s liver was perfused through portal vein before it was harvested,and the anastomosis was modified as continous suture with one suture for the suprahepatic inferior vena cava. Two hundred and ten orthotopic liver transplantations were performed in Wistar male rats according to this method. Results The mean time of donor operation was 35 min, and that of recipient operation was 51 min. The mean cold preserving time of graft was 60 min.The anhepatic phase was about 17 min 〔(17.6±4.5) min〕. Nineteen rats died during operation. The causes of death included: bleeding of suprahepatic inferior vena cava, infrahepatic vena cava thrombosis, biliary obstruction, portal vein constriction and thrombosis, liver injury, bleeding of left subphrenic vein, infection, excessively deep anesthesia and respiratory failure. The 24 hour survival was 91.0%(191/210),and the one week survival was 85.2%(179/210).Conclusion Through the modification of the anastomosis of suprahepatic inferior vena cava, the nonhepatic time of the recipients could be shortened,and the complications could be decreased. In this way, the survival of recipient rats after liver transplantation could be increased.