正常妊娠外周血组织多肽抗原与完整hCG关系的探讨

Study on the relation between tissue polypeptide antigen and intact hCG levels in peripheral blood in normal pregnancy women.

目的 :测定正常妊娠妇女外周血中组织多肽抗原 (TPA)和完整hCG的浓度 ,探讨两者变化的关系。方法 :使用TPA酶免试剂盒与双抗体夹心法定量测定血清中TPA的含量。应用时间分辨荧光免疫分析法 (time resolvedfluorescenceimmunoassay)测定hCG的浓度。结果 :正常妊娠期外周血TPA的浓度随妊娠进展逐步增加 ,晚孕期最高。与早、中孕期及非孕期比较差异均有显著性 (P <0 .0 5 )。血清完整hCG浓度早孕期明显高于其它各期 (P <0 .0 5 )。结论 :外周血TPA水平随妊娠进展逐步增加可能代表滋养细胞凋亡的情况 ;hCG浓度与滋养细胞增殖相关。TPA、hCG对预测某些病理妊娠可能有帮助。

Objective:To study the relation between tissue polypepti de antigen and intact hCG,and to dertermine the concentrations of TPA and hCG in the peripheral blood of normal pregnancy women.Methods:TPA serum EIA kit was used to quantitate the TPA in the serum based on the ELISA principl es.The level of hCG was dertermined with time-resolved fluorescence immunoassay . Results:With the gestation increasing,the level of TPA was incre asing step by step,and reached the peak in the third trimester compared with the first,second trimester and nonpregnancy, the level of TPA in the third trimest er had obviously difference(P<0.05).The l evel of hCG was higher in the first trimester than those of the others(P <0.05).Conclusion: With the gestation progressing, the increased level of TPA in peripheral blood may reflect the apoptosis of the trophoblast cells and the level of hCG relates to trophoblast cells proliferation,which may be helpful for predicting pathologic pregnancy with dysfunction of the trophoblast cells.