期刊文献+

高效液相色谱-紫外法测定指血中苯丙氨酸与酪氨酸浓度 被引量:4

Determination of phenylalanine and tyrosine in finger blood by high-performance liquid chromatography with untraviolet detection.
下载PDF
导出
摘要 目的 探讨采用高效液相色谱法测定微量指血中苯丙氨酸和酪氨酸的可行性。方法 指血标本经去蛋白后取上清 ,用反相高效液相色谱 -紫外法进行检测。结果 苯丙氨酸线性范围为 6~ 1 5 1 2 μmol/L ,最低检测浓度为1 5 μmol/L ;平均回收率为 98 2 % ,批内CV为 3 2 % ,批间为 5 4 %。酪氨酸的线性范围为 5 5~ 1 2 5 0 μmol/L。最低检测限为 1 0 μmol/L ,平均回收率为 98 8% ,批内CV为 2 6 % ,批间为 4 6 %。 1 0 2例健康儿童指血苯丙氨酸浓度为 (6 7 76 4~ 1 5 4 3) μmol/L ,酪氨酸浓度为 (6 2 2 9± 1 3 97) μmol/L ,Phe/Tyr比值为 (1 1 5 4~ 0 .2 7)。结论 本法灵敏度好 ,特异性高 ,方法简便快速 ,适合于指血中Phe和Tyr的同时测定。 Objective: To investigate the feasibility of determination of phenylalanine and tyrosine in finger blood by reverse phase high-performance liquid chromatography(RP-HPLC) method. Methods: Supernatant fluid of finger blood precipitated with perchloric acid was isocratically eluted using a base-deactivated C 8 column with 5% acetonitrile in water as the mobil phase. Untraviolet detector worked at 210nm. Results: Good linearity was observed in the range of 6~1512 μmol/L for phenylalanine, the lower limit of detection was 1.5μmol/L, the average recovery was 98.2%, and the intra-and inter-assay coefficients of variation (CV) were 3.2% and 5.4% respectively. There also was a good linearity in the range of 5.5~1250μmol/L for tyrosine. The average recovery was 98.8%, the intra-and inter-assay coefficients of variation (CV) were 2.6% and 4.6% respectively. The lower limit of detection was 1.0μmol/L.Phenylalanine and tyrosine in finger blood of 102 health enfant were (67.76±15.43)μmol/L and (62.29±13.97)μmol/L respectively. The ratio of phe/tyr was 1.15±0.27.Conclusion:This method is sensitivity, accurate, rapid and simple in measuring phenylalanine and tyrosine in finger blood.
出处 《中国优生与遗传杂志》 2005年第3期29-31,共3页 Chinese Journal of Birth Health & Heredity
基金 湖南省科技厅资助项目 (0 4SK3 0 5 2 )
关键词 指血 苯丙氨酸 酪氨酸 紫外法 高效液相色谱 健康儿童 血标本 平均回收率 酸浓度 微量 High-performance liquid chromatography Phenylalanine Tyrosine Finger blood
  • 相关文献

参考文献7

二级参考文献17

共引文献492

同被引文献63

  • 1唐爱国,洪敏,莫喜明,陈若虹.两种血清苯丙氨酸高效液相色谱测定法的比较[J].中国实验诊断学,2005,9(4):607-609. 被引量:6
  • 2张丹,陈红.HPLC法测定甘氨酰-L-酪氨酸含量及其有关物质[J].药物分析杂志,2006,26(5):664-666. 被引量:6
  • 3GuoS YangFM ZhangL.The synthesis of herbicide sulcotrione.农药,2001,40(7):20-22.
  • 4WuNX YuZB ZhuY ZhangX.Influence of herbicides on the serum glucose level in rats.毒理学杂志,2006,20(2):107-108.
  • 5Hall MG, Wilks MF, Provan WM, Eksborg S, Lumholtz B. Pharmacokinetics and pharmacodynamics of NTBC (2-(2-nitro-4-fluoromethylbenzoyl)-1,3-cyclohexanedione) and mesotrione, inhibitors of 4-hydroxyphenyl pyruvate dioxygenase(HPPD) following a single dose to health male volunteers[J]. Br J Clin Pharmacol, 2001, 52(2):169-177.
  • 6Schulz A, Ort O, Beyer P, Kleinig H. SC-0051, A 2-benzoyl-cyclohexane-1,3-dione bleaching herbicide, is a potent inhibitor of the enzyme p-hydroxyphenylpyruvate dioxygenase[J]. FEBS Lett, 1993, 318(2):162-166.
  • 7Secor J. Inhibition of barnyardgrass 4-hydroxyphenylpyruvate dioxygenase by sulcotrione[J]. Plant Physiol, 1994, 106(4):1429-1433.
  • 8Lock EA, Gaskin P, Ellis MK, Provan WM, Robinson M, Smith LL, et al. Tissue distribution of 2-(2-nitro-4-trifluoromethylbenzoyl)cyclohexane-1-3-dione (NTBC): effect on enzymes involved in tyrosine catabolism and relevance to ocular toxicity in the rat[J]. Toxicol Appl Pharmacol, 1996, 141(2):439-447.
  • 9Ellis MK, Whitfield AC, Gowans LA, Auton TR, Provan WM, et al. Inhibition of 4-hydroxyphenylpyruvate dioxygenase by 2-(2-nitro-4trifluoromethylbenzoyl) cyclohexane1, 3-dione and 2-(2-nitro-4-methanesulphonyl benzoyl)-cyclohexane1, 3-dione[J]. Toxicol Appl Pharmacol, 1995, 133(1):12-19.
  • 10Gao J Z. Pak. J. Biol. Sci. , 2005, 8(4) : 512 -519.

引证文献4

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部