摘要
以精选冬小麦(Triticum aestivum L.)4185 种子为试验材料,采用 CaCl2、EGTA、LaCl3及 Verapamil配制的溶液处理萌发 24 h 的小麦根系,研究其胚芽鞘及根系对渗透胁迫的不同反应。结果表明,PEG-6000(20%)诱导的渗透胁迫下,低浓度 Ca2+(10-6~10-3 mol·L-1)处理小麦根系时,不同浓度处理间胚芽鞘及根系的生长无显著差异,10-2 mol·L-1 的 Ca2+则抑制胚芽鞘和根系的生长;EGTA(1、5、10 mmol·L-1)、LaCl3(0.5、1 mmol·L-1)和Verapamil(250、500 μmol·L-1)处理根系时,均抑制了胚芽鞘和根系的生长,这种抑制效应与提高过氧化物酶(POD)活性有密切关系。表明在渗透胁迫下,小麦对缺钙更敏感,高浓度 Ca2+以及减少细胞外 Ca2+和阻断 Ca2+的运转,均对生长有抑制作用。
Under osmotic stress induced by PEG-6000 (20%), when the roots of wheat(Triticum aestivum L. cv 4185)were treated with low concentration of exogenous Ca2+ at 10-6-10-3 mol·L-1, there were no significant difference in growth of coleoptiles and roots, but the growth was inhibited by exogenous Ca2+ at 10-2 mol·L-1. The treatments for roots with EGTA (1, 5,10 mmol·L-1), LaCl3 (0.5, 1 mmol·L-1) and Verapamil (250, 500 μmol·L-1) inhibited the growth of coleoptiles and roots, which were closely related to the increase of peroxidase (POD) activity. These results showed that under osmotic stress, the wheat was more sensitive to deficiency of Ca2+. The higher exogenous Ca2+ concentration, lower extracellular Ca2+ concentration and blocking Ca2+ transfer would suppress the growth of wheat coleoptiles and roots.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第3期486-491,共6页
Scientia Agricultura Sinica
基金
河北省博士资金资助项目(00547001D-1)