血管内皮生长因子C对舌鳞癌癌周淋巴管的促增殖作用

Interaction of vascular endothelial growth factor-C over-expression tongue squamous cell carcinoma cell line Tca8113 with peri carcinoma lymphatics

目的研究血管内皮生长因子C(VEGF-C)对舌鳞癌癌周淋巴管的促增殖作用.方法将VEGF-C高表达的舌鳞癌细胞株(A组)接种于发育至第6～8天的鸡胚绒毛尿囊膜(CAM),设立空白质粒转染组(B组)和空白对照组(C组),5′-核苷酸酶(5′-Nase)染色观察瘤周淋巴管,淋巴管形态学测量统计分析淋巴管数密度(LVD)、截面面积以及周长的变化.结果 3组在CAM上均能成瘤,肿瘤组织对周围CAM的侵袭较弱,瘤体组织内未见淋巴管的生成;瘤周淋巴管形态学测量分析发现A组癌周淋巴管的LVD、截面面积和周长均高于B、C组,差异有统计学意义(P<0.01);B、C组之间差异无统计学意义(P>0.05).结论 CAM是研究淋巴管的较理想的模型;VEGF-C能够诱导癌周淋巴管扩张,这可能是VEGF-C增加舌鳞癌颈淋巴转移的机制之一.

Objective To detect the role of Vascular endothelial growth factor C(VEGF C) in the interaction of tongue squamous cell carcinoma (TSCC) with the peri carcinoma lymphatics.Methods TSCC cells were implanted on the chorioallantoic membrane (CAM), in which, group A was transfected by plasmid pREP7 including VEGF C, group B was transfected by plasmid pREP7, and group C was Tca8113. Lymphatic vessels were stained by 5′ Nase.Morphologic analysis was used to evaluate the alteration of lymphatic vessels density (LVD), area and perimeter.Results The transplanted tumor well on CAM. The expression of VEGF C in group A was higher than that in control group B and C. The LVD, perimeter and area in group A were (5 3±0 41)/high power field, (148±21) μm and (76 8±13 5)μm 2 respectively ingroup A, which were significantly higher than that of group B and C ( P <0 01), while there were no significant difference between group B and C.Conclusions CAM is ideal model for the research of lymphatic vessels; Over expression of VEGF C could induce the dilatation and LVD increase of peri tumor lymphatic vessels, which maybe one of the mechanisms that VEGF C metastasis of TSCC through lymphatic vessels.