大豆疫霉根腐病菌毒素的提取及生物活性测定

Study on Extraction and Bioactivity Assay of Pathotoxin Produced by Phytophthora sojae

对大豆疫霉根腐病原菌的主要致病因子之一—毒素的提取方法进行了探讨,并用抗感不同大豆品种的切根苗进行了毒素生物活性及最佳浓度的测定、筛选。结果表明:毒素的提取采取弱极性的有机溶剂萃取的方法比较理想,尤其是用乙醚萃取;用稀释100×浓度的毒素处理抗感不同的大豆品种的切根苗可以产生与病原菌下胚轴接种法相同的症状。

In this paper, the extraction and bioactivity assay of pathotoxin produced by Phytophthora sojae (Pmg.) were systematically researched. The main results were as follows: The best extraction way of the pathotoxin produced by Pmg was to use the weak polarity organic solvent, especially ether. The rude crystalline of the pathotoxin was gotten by continuous extraction by ether; The bio-activity of the rude pathotoxin was determined by the seedlings with cutting roots, and the symptom of seedlings with cutting roots treated with the concentration of the dilute factor of 100 appeared the same as those with the hypocotyl inoculation. This also proved that the pathotoxin was a kind of Non- Host Specitiy Toxin.